湖南中医药大学学报2013，Vol.33Issue(5)：24-29,48,7.DOI:10.3969/j.issn.1674-070X.2013.05.007.024.06
肝阳上亢与肝阳化风证大鼠模型脾淋巴细胞的蛋白质组学分析
Proteomics analysis of spleniclymphocytes in SD rats with liver-yang hyperactivity syndrome and liver-yang forming wind syndrome
摘要
Abstract
Objective To establish the two-dimensional gel electrophoresis (2-DE) map of the spleniclymphocytes in animal models of hypertensive with liver-yang syndrome,hemorrhagic and ischemic stroke with liver-yang forming wind syndrome,and then to elucidate the essence of the two syndromes by analyzing the identical and differential proteins between them.Methods The SD rats were randomly divided into 4 groups including the normal control group,hypertensive with liver-yang hyperactivity syndrome group,hypertensive intracerebral hemorrhage (HICH) and cerebral infarction (CI) with liver-yang forming wind syndrome group.Models were evaluated by their general conditions and behavior,the blood pressure measurement and rotating time,histological morphology and biological behavior,and the TTC staining.The spleniclymphocytes were separated by 2-DE and analyzed by PDQuest software.The target protein spots were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS).Results Beta-actin and other 3 protein dots were up-regulated in all the three groups as compared with healthy control,more obviously up-regulated in liver-yang forming wind syndrome group than in liver-yang hyperactivity syndrome group.Compared with the healthy control,Annexin Ⅲ and other 2 protein dots were down-regulated in the three groups,more obviously down-regulated in liver-yang forming wind syndrome group than in liver-yang hyperactivity syndrome group.There were no significant differences between HICH and IC with liver-yang forming wind syndrome groups in the expression levels of Annexin Ⅲ and other 7 protein dots.NADH dehydrogenase was down-regulated in IC with liver-yang forming wind syndrome group as compared with HICH with liver-yang forming wind syndrome group.Conclusion There are identical and differential proteins between liver-yang hyperactivity syndrome and liver-yang forming wind syndrome,which suggests the two syndromes have the same material base but different essence.关键词
蛋白质组学/大鼠/肝阳上亢/肝阳化风/脾淋巴细胞/实验研究Key words
proteomics/ SD rats/ liver-yang hyperactivity syndrome/ liver-yang forming wind syndrome/ spleniclymphocytes/ experiment research分类
医药卫生引用本文复制引用
张扬,熊新贵,陈疆,梁清华,吴丹,祝皓,张永..肝阳上亢与肝阳化风证大鼠模型脾淋巴细胞的蛋白质组学分析[J].湖南中医药大学学报,2013,33(5):24-29,48,7.基金项目
国家自然科学基金重大研究计划资助项目(90409002) （90409002）
湖南省自然科学基金项目(06JJ20012) （06JJ20012）
湖南省重点学科、国家中医药管理局重点学科经费资助. （）
