湖南中医药大学学报2013，Vol.33Issue(5)：65-66,74,3.DOI:10.3969/j.issn.1674-070X.2013.05.018.065.02
Folin-Ciocalteu比色法测定安化黑茶中茶多酚的含量
Determination of the total polyphenol in Anhua dark-tea by Folin-Ciocalteu colorimetry
摘要
Abstract
Objective To establish a method for determination of the content of total polyphenols in Anhua dark-tea.Methods According to GB/T 8313-2008,the total polyphenol content in Anhua dark-tea was analyzed by spectrophotometry with Folin-Ciocalteu reagent and Gallic acid used as standards.The optimal determination conditions could be calculated according to the absorption at 765 nm by reacting 5 mL 0.2 mol/L Folin-Ciocalteu with 4 mL 7.5％ Na2CO3 for an hour at 25 ℃.Results In this method,the concentration of Gallic acid showed a good linearity with absorbance at the range of 0.49 to 4.9 μg/mL,the average recovery was 98.0％,and RSD was 1.4％.The total polyphenols content in Anhua dark-tea was 2.49％ and RSD was 1.3％.Conclusion The method was accurate and reliable for the determination of total polyphenol in Anhua dark-tea.关键词
安化黑茶/茶多酚/Folin-Ciocalteu试剂/分光光度法/含量测定Key words
Anhua dark-tea/ total polyphenol/ Folin-Ciocalteu reagent/ spectrophotometry/ content determination分类
医药卫生引用本文复制引用
罗堃,何群,葛云鹏,谭琥,邹龙,彭思源,刘朝勇..Folin-Ciocalteu比色法测定安化黑茶中茶多酚的含量[J].湖南中医药大学学报,2013,33(5):65-66,74,3.基金项目
湖南中医药大学校企合作科研项目(50010026). （50010026）
