华西口腔医学杂志2013，Vol.31Issue(3)：242-246,5.DOI:10.7518/hxkq.2013.03.007
4种牙科金属材料对成纤维细胞L929凋亡相关基因及蛋白表达的影响
Effects of four dental alloys on apoptosis related gene and protein expression of fibroblast L929
摘要
Abstract
Objective To investigate the effects of the leaching liquids of 4 differents kinds of dental alloys (Au alloy,Ag-Pd alloy,Co-Cr alloy,Ni-Cr alloy) on apoptosis related gene and protein of fibroblast L929.Methods The L929 cells of mouse were treated in vitro with leaching liquids of 4 different kinds of dental alloys,Au alloy (group A),Ag-Pd alloy(group B),Co-Cr alloy(group C) and Ni-Cr alloy(group D).The RPMI1640 cell medium containing 10％ fetal calf serum was served as a control (group E).The effects of these alloys on the expression of caspase-3,8,9 of L929 cells were examined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry method.Results After 48 h culture,the mRNA levels of caspase-3 and caspase-9 demonstrated significant differences between the groups expect group A and group E.The mRNA levels of caspase-8 had no change in all groups.The expression of caspase-3 and caspase-9 were significant differences between the groups expect group A and C,group B and D.The expression of caspase-8 had no change in all groups.Conclusion The leaching liquids of 4 different kinds of dental alloys expect Au alloy may induce cell apoptosis through mitochondrion pathway.关键词
牙科金属/浸提液/成纤维细胞L929/凋亡/免疫组织化学Key words
dental alloy/leaching liquid/fibroblast L929/apoptosis/immunohistochemistry分类
医药卫生引用本文复制引用
孟贺,丁洁,李任,梁锐英,吴文慧..4种牙科金属材料对成纤维细胞L929凋亡相关基因及蛋白表达的影响[J].华西口腔医学杂志,2013,31(3):242-246,5.基金项目
唐山市科技局指令性基金资助项目(12140209A-39) （12140209A-39）
河北联合大学青年基金资助项目(Z201142) （Z201142）
