华西口腔医学杂志2013，Vol.31Issue(3)：294-299,6.DOI:10.7518/hxkq.2013.03.019
两种多孔生物玻璃支架材料的体外细胞相容性研究
Cytocompatibility of two porous bioactive glass-ceramic in vitro
摘要
Abstract
Objective To compare the cytocompatibility of two kinds porous bioactive glass-ceramic made by same raw materials.Methods Apatite/wollastonite bioactive glass-ceramic (4006) were prepared by sol-gel method,and bioactive glass (45S5) were prepared by melting method.Bone marrow stromal cells (BMSCs) were cultivated,differentiated and proliferated into osteoblasts,from a rabbit's marrow in the differentiation culture medium with active function.The viability of BMSCs cultivated with extraction of these two kinds of biomaterial,which could represent the cytotoxicity effect of 4006 and 45S5 against BMSCs,was evaluated by the MTT assay.BMSCs were seeded and cocultivated with two kinds of biomaterial scaffolds respectively in vitro.The proliferation and biological properties of cells adhered to scaffolds were observed by inverted phase contrast microscope,scanning electron microscope (SEM),and environmental scanning electron microscope (ESEM),and a suitable cell amount for seeding on the scaffold was searched.Results There was no difference on the viability of BMSCs only cultured for one day by complete extract of 4006 and culture medium (P＞0.05),but there was significant difference between them when the cells had been cultured for 3 days (P＜0.01).The extract of 45S5 had significantly higher cytotoxicity than extract of culture medium (P＜0.01).The BMSCs adhered,spread,and proliferated throughout the pores of the scaffold 4006,and the amount of cells adhered to 4006 was more than to 45S5.The adhered cells to 4006 increased with the rising amount of cells seeded.And 2×107cells·mL-1 suspension resulted in the highest cell adherence during the comparative cell adherence test.Conclusion Apatite/wollastonite bioactive glass-ceramic has good bioactivity and cytocompatibility.Therefore,it may have the potential to be a new cell vehicle for bone tissue engineering.And the suitable seeding cell amount of apatite/wollastonite bioactive glass-ceramic should be 2×107cells·mL-1 or even more than that.关键词
骨组织工程/支架/生物活性微晶玻璃/溶胶-凝胶法/骨髓基质细胞/细胞相容性Key words
bone tissue engineering/scaffold/apatite/wollastonite bioactive glass-ceramic/sol-gel method/bone marrow stromal cells/cytocompatibility分类
医药卫生引用本文复制引用
章燕,蒋欣泉,张秀丽,王德平,甄蕾..两种多孔生物玻璃支架材料的体外细胞相容性研究[J].华西口腔医学杂志,2013,31(3):294-299,6.基金项目
上海市青年医师培养计划基金资助项目(人才-2012-04-03) （人才-2012-04-03）
上海市科委基础研究重点基金资助项目(12JC1408500) （12JC1408500）
