四川大学学报（医学版）2013，Vol.44Issue(4)：522-525,530,5.
非分泌型CXCL16在乳腺癌细胞系中的表达及对其生物学特性的影响
Expression of Non-secretory CXCL16 and Its Impact on Biological Characteristics in Breast Cancer Cell Lines
摘要
Abstract
Objective To detect the expression of non-secretory CXCL16 and its impact on malignant biological behaviors in breast cancer cell lines.Methods RT-PCR was carried out to examine the expression of CXCL16 mRNA in breast cancer cell lines with different aggressiveness SK-BR-3,MCF-7,MDA-MB-231,MDAMB-435S and human mammary normal epithelial celll MCF-10A.The eukaryotic expression plasmid of CXCL16 was transfected into MDA-MB-231 cells and overexpression of CXCL16 was confirmed by Real time PCR and Western blot.Boyden Chamber assay was used to determine cell migration and invasion,while MTT assay was performed to determine cell proliferation.Results Among four breast cancer cell lines,CXCL16 mRNA was highly expressed in MCF-7,lowly expressed in MDA-MB-231,while MCF-10A faintly expressed CXCL16.Overexpression of CXCL16led to a decrease in cell migration,invasion but not proliferation in MDA-MB-231 cells.Conclusion Expression of non-secretory CXCL16 is associated with aggressiveness of breast cancer cell lines,and CXCL16 expression inhibits cell migration and invasion in vitro.关键词
CXCL16/MDA-MB-231/迁移/侵袭/增殖Key words
CXCL16/MDA-MB-231/Migration/Invasion/Proliferation引用本文复制引用
方业颖,雷倩倩,李燕,陈念永..非分泌型CXCL16在乳腺癌细胞系中的表达及对其生物学特性的影响[J].四川大学学报（医学版）,2013,44(4):522-525,530,5.基金项目
四川省科技支撑计划项目(No.2009SZ0146)资助 （No.2009SZ0146）
