四川大学学报（医学版）2013，Vol.44Issue(4)：536-539,544,5.
聚乙二醇修饰对苦瓜核糖体失活蛋白的肝细胞毒性影响
Effect of PEGylation of α-Momorcharin against Its Hepatotoxicity in Rats
摘要
Abstract
Objective To explore the effect of PEGylation of α-Momorcharin (α-MMC),one of ribosomeinactivating proteins from bitter melon seed,against its hepatotoxicity in rats.Methods SD rats were randomized into NS group,α-MMC treated groups,and α-MMC-PEG treated groups.The doses of α-MMC and α-MMC-PEG were high,middle,and low dose(6.25,2.08,0.70 mg/kg).The rats were given different dose of α-MMC,or α-MMC-PEG via caudal vein every other day for consecutive 28 days and then left for 14 days recovery.The general condition of animals was observed,blood and liver samples were collected for liver function study and pathological examination on day 28 after initiation of administration and on day 14 after withdrawal.Results On day 28 after initiation of administration,the liver function damages were found in high-dose and middle-dose of α-MMC treated groups,such as the decreasing of ALB,increasing of GLB,A/G ratio decreasing and the dose-dependant increasing of AST,BIL and CHO.The pathological changes of hepatotoxicity were also observed in these two groups,including the massive hepatocyte,swelling degeneration,inflammatory cell infiltration,congestion and diffusive necrosis.However,the liver function and pathological changes in α-MMC-PEG treated groups were betterthan those in α-MMC treated groups.Conclusion PEGylation could reduce the hepatotoxicity of α-MMC to rats.关键词
α型苦瓜核糖体失活蛋白/PEG修饰/肝细胞毒性Key words
α-Momorcharin/PEGylation/Rat hepatotoxicity引用本文复制引用
金家贵,孟延发,郭卫,郑崛村,沈富兵..聚乙二醇修饰对苦瓜核糖体失活蛋白的肝细胞毒性影响[J].四川大学学报（医学版）,2013,44(4):536-539,544,5.基金项目
四川省教育厅资助科研项目(09ZC084,08ZA096)资助 （09ZC084,08ZA096）
