基础医学与临床2013，Vol.33Issue(6)：648-654,7.
DJ-1可通过增强自噬水平缓解鱼藤酮致MN9D细胞损伤
DJ-1 alleviates rotenone-induced cell injuries via enhancing autophaty in MN9D cells
摘要
Abstract
Objective To explore the protective effect of DJ-1 on MN9D cells and its mechanisms.Methods Transiently transfected wild type D J-1 into MN9D cells.The MN9D cell viability and cell apoptosis level were observed by MTT assay and flow cytometry respectively.The numbers of LC3-positive puncta were examined by confocal images.The ratio of LC3 Ⅱ to LC3Ⅰ was detected via Western blot.Resuts DJ-1 overexpressing could attenuate the decreased cell viability induced by rotenone treatment (P ＜ 0.05).The increase of apoptosis caused by rotenone was diminished by overexpression of D J-1 (P ＜ 0.05).Overexpressing of DJ-1 increased the numbers of LC3 positive puncta(P ＜ 0.001) and the ratio of LC3Ⅱ to LC3Ⅰ (P ＜ 0.001) in MN9D cells treated with rotenone.Giving 3-MA did make the numbers of LC3 positive puncta down to the basal level and reduce the ratio of LC3-Ⅱ / Ⅰ (P ＜ 0.05).Meanwhile,giving autophagy inhibitor 3-MA,the protective effects of D J-1 on MN9D cells were disappeared.Conclusions DJ-1 upregulated level of autophagy can alleviate the toxication caused by rotenone in MN9D cells.关键词
自噬/MN9D细胞/鱼藤酮/3-MAKey words
autophagy/ MN9D cells/ rotenone/ 3-M分类
医药卫生引用本文复制引用
刘慧丰,杨巍巍,高华,杨慧..DJ-1可通过增强自噬水平缓解鱼藤酮致MN9D细胞损伤[J].基础医学与临床,2013,33(6):648-654,7.基金项目
国家重点基础研究发展计划(2011CB504102,2012CB722407) （2011CB504102,2012CB722407）
国家自然科学基金(30970940) （30970940）
北京市自然基金(7131001) （7131001）
