基础医学与临床2013，Vol.33Issue(6)：704-707,4.
保存温度和时间对糖化血红蛋白A1c样本稳定性的影响
Stability of haemoglobin A1e in different Storage temperature and time
摘要
Abstract
Objective To investigate the stability time of HbA1c samples in 4 different storage temperature conditions.Methods 20 samples with different HbA1 c levels were collected.Each sample is thoroughly mixed and distributed into 45 parts.In each level group,a random selected sample was measured immediately,and the remaining samples were immediately grouped and stored at room temperature(22 ～ 25 ℃),refrigerated (2 ～ 8 ℃),frozen (≤-20 ℃),cryopreservation (≤-70 ℃),which were measured at 1,2,3,7,15,20 day and 1,2,3,6 month and 1y respectively.HbA1c levels were detected by HPLC method.The changes of average level of HbA1c versus time with 4 different temperatures were observed and the stability of HbA1 c was evaluated by different method or judgment.Results Based on a defined bias less than 6％,HbA1c was stable for one year at-70 ℃ ;HbA1 c in low concentration levels was stable for 7 days at-20 ℃,while in high concentration levels could be stable for 20 days with 11.2％ average annual decline rate.HbA1c at room temperature and refrigerated storage conditions could be stable for 3 days,till 7 days,HbA1c level dropped by average-9.1％ and-7.1％ respectively.Conclusions This study provides a basis to formulate and improve HbA1c specimens preservation conditions.The Cryopreservation is more favorable for large-scale epidemiological studies of HbA1 c.关键词
糖化血红蛋白A1c/稳定性Key words
HbA1c/ Stability分类
医药卫生引用本文复制引用
迟书玲,邱玲,郝英英,张麟,赵芳,徐二木,陈雨,朱广瑾..保存温度和时间对糖化血红蛋白A1c样本稳定性的影响[J].基础医学与临床,2013,33(6):704-707,4.基金项目
国家科技部科技基础性专项重点项目(2006FY110300) （2006FY110300）
