基础医学与临床2013，Vol.33Issue(6)：744-748,5.
马兜铃酸通过ERK1/2信号传导途径诱导人脐静脉血管内皮细胞凋亡
Aristolochic acid induces apoptosis of human umbilical vein endothelial cells by suppressing the ERK1/2 signaling pathway
石红 1冯江敏2
作者信息
- 1. 抚顺市第二医院肾内科,辽宁抚顺113001
- 2. 中国医科大学附属第一医院肾内科,辽宁沈阳110001
- 折叠
摘要
Abstract
Objective To make sure whether the ERK1/2 signaling pathway plays an important role in aristolochic acid (AA)-induced the apoptosis of human umbilical vein endothelial cells (HUVECs).Methods Cell viabilities were determined by the MTT assays.Apoptotic morphologic changes of the treated cells were observed under fluorescence microscope.The proportions of apoptosis were assessed by flow cytometry.Western blot was used to evaluate ERK1/2 phosphorylation.Results The MTT results showed that AA significantly decreased the viabilities of HUVECs in a dose-and time-dependent manner.The flow cytometry showed that AA significantly increased the apoptosis of HUVECs in a dose-dependent manner.Microscopic apoptotic changes were also observed in these cells.Furthermore,AA inhibited ERK1/2 activation,which,however,was attenuated when the cells were pretreated with PD98095.An increase of apoptosis induced by AA was inhibited when the cells were pretreated with PD98095.Conclusions AA induces apoptosis of HUVECs via the ERK1/2 signaling pathway.关键词
马兜铃酸/内皮细胞/凋亡/ERK1/2信号传导途径Key words
aristolochic acid/ endothelial cell/ apoptosis/ ERK1/2 signaling pathway分类
医药卫生引用本文复制引用
石红,冯江敏..马兜铃酸通过ERK1/2信号传导途径诱导人脐静脉血管内皮细胞凋亡[J].基础医学与临床,2013,33(6):744-748,5.
