基础医学与临床2013，Vol.33Issue(6)：749-751,3.
盐酸苯肼诱发小鼠网织红细胞条件的优化
Optimization of the conditions for inducing mouse reticulocyte by hytrazinobenzene hydrochloride
曹玲玲 1何超 1薛建有 1毛群铨 1施鸣铭 1戚武林 1张世馥1
作者信息
- 1. 浙江理工大学生命科学学院蛋白质组学与分子酶学研究室,浙江杭州310018
- 折叠
摘要
Abstract
Objective To choose the optimum conditions for inducing mouse reticulocytes by using hytrazinobenzene hydrochloride.Methods One percent hytrazinobenzene hydrochloride was injected into back subcutaneous tissue of the ICR and BALB/c mouse everyday.The dose was100,200,300 and 400 μL,respectively for 9 days.Then tail vein blood was sampled and examined every day.After staining the reticulocyte induced efficiency was statistical analyzed by using the bright tar blue staining.Results The morphology and proportion of reticulocytes were the best when the mice were injected 200 μL hytrazinobenzene hydrochloride for 4 days.The ratio of induced reticulocyte was about 83.16％ ±3.41％.The cell morphology of reticulocytes was irregular,the state of cells was poor and animals began to die as well when mice were injected 300 μL or 400 μL hytrazinobenzene hydrochloride.Conclusions The best conditions for inducing reticulocyte are as follows:weight 25 to 35 g mice daily injection of 200 μL 1％ hytrazinobenzene hydrochloride for 4 days.关键词
网织红细胞/盐酸苯肼/诱发条件Key words
reticulocyte/ hytrazinobenzene hydrochloride/ induced conditions分类
生物科学引用本文复制引用
曹玲玲,何超,薛建有,毛群铨,施鸣铭,戚武林,张世馥..盐酸苯肼诱发小鼠网织红细胞条件的优化[J].基础医学与临床,2013,33(6):749-751,3.
