基础医学与临床2013，Vol.33Issue(7)：799-803,5.
钙激活中性蛋白酶介导雌激素增强MCF-7乳腺癌细胞的存活力
Calpain mediates estrogen-enhanced survival in MCF-7 breast cancer cells
摘要
Abstract
Objective To investigate the effects of estrogen (E2) on the gene expression of calpainl and calpain activity,and the possible role of calpain in E2-enhanced survival in breast cancer cells.Methods Human breast cancer cell line MCF-7 was employed as a model system.RT-PCR was used to analyze the level of mRNA expression and western blotting was employed to access proteolysis of FAK.Serum starvation was used to induce cell death,Trypan blue staining and cell counting were used to evaluate cell viability.Results Stimulation of MCF-7cells with E2 increased mRNA expression of calpainl and calpain activity.E2 also increased cell viability by 14.29％ (P ＜ 0.05) during serum starvation,while calpain inhibitors significantly suppressed the E2-increased expression of calpainl mRNA,calpain activity,and cell viability by 19.17％ (P ＜ 0.05).Conclusions E2 increased expression of calpainl mRNA and calpain activity which may contribute to the E2-stimulated survival during serum starvation in MCF-7 cells.关键词
雌激素/钙激活中性蛋白酶/基因表达/细胞存活/乳腺癌细胞Key words
estrogen/ calpain/ gene expression / cell survival/ breast cancer cells分类
医药卫生引用本文复制引用
刘晓红,王筑婷,李阳,杨莉,雷霆雯,王旭东..钙激活中性蛋白酶介导雌激素增强MCF-7乳腺癌细胞的存活力[J].基础医学与临床,2013,33(7):799-803,5.基金项目
国家自然科学基金(30860093) （30860093）
贵州省教育科技优秀人才专项基金[黔省专合字(2008)56号)] （2008）
