基础医学与临床2013，Vol.33Issue(7)：804-807,4.
葡萄糖通过mTORC1信号通路调控成纤维细胞增殖
Glucose controls the fibroblast proliferation through mTORC1 signal pathway
摘要
Abstract
Objective To study the effect of glucose on the mouse skin fibroblast proliferation.Methods The cells cultured with different concentrations of glucose medium including 5.5 mmol/L,15 mmol/L,25 mmol/L,and then tested the cells proliferation by MTT.The formation of translation initiation factor 4F complex was analyzed by 7-MGTP pulldown test and the mTORC1 signal pathway was analyzed by Western blot.Results In contrast with 5.5 mmol/L,when the concentration of glucose medium was 15 mmol/L,it promoted the cell proliferation and the translation initiation,activated the mTORC1 signal pathway.25 mmol/L inhibited the cell proliferation and the translation initiation,inactivated the mTORC1 signal pathway (included the 4EBP1 related with cell proliferation and the Akt related with cell survival),but the S6K1 was over activatied.Conclusions Glucose controls fibroblast proliferation through the mTORC1 signal pathway.关键词
慢性难愈创伤/葡萄糖/成纤维细胞/真核翻译起始复合物/哺乳动物雷帕霉素靶蛋白复合物1Key words
chronic wound healing/ glucose/ fibroblast/ eIF4F/ mTORC1分类
医药卫生引用本文复制引用
宋娇,吴登艳,邢伟,郝进,徐祥,黄宏..葡萄糖通过mTORC1信号通路调控成纤维细胞增殖[J].基础医学与临床,2013,33(7):804-807,4.基金项目
国家自然科学基金(30970651) （30970651）
