基础医学与临床2013，Vol.33Issue(7)：854-858,5.
反义锁核酸在转基因小鼠体内阻断乙肝病毒C基因表达
Inhibition of hepatitis B virus(HBV) replication using antisense LNA targeting to C gene in HBV transgenetic mice
摘要
Abstract
Objective To investigate the inhibitory effects on hepatitis B virus (HBV) replication of antisense locked nucleic acid (LNA) targeting to C gene in transgenic mice.Methods Antisense LNA were injected into transgenic mice via the tail vein.Serum HBV DNA was tested by real-time PCR.Serum HBeAg was tested by timeresolved fluorescence immune assay.The expression of HBcAg in the liver was detected by immuneohistochemistry.Results Five days after LNA injection,serum HBV DNA level in the AS-LNA group was reduced by 44.47％,and serum HBeAg level was decreased by 63.46％.These values were significantly higher than those in the control groups (all P ＜ 0.05).The expression of HBcAg in liver was significantly lower than that in control groups.Conclusions Antisense LNA targeting to C gene has strong inhibitiory effect on HBV replication and expression in transgenic mice and C gene is a potentical target as gene therapy.关键词
反义锁核酸/乙型肝炎病毒/转基因小鼠/半乳糖配体Key words
locked nucleic acid/ Hepatitis B virus/transgenic mice/ galactose ligand分类
医药卫生引用本文复制引用
邓益斌,温旺荣..反义锁核酸在转基因小鼠体内阻断乙肝病毒C基因表达[J].基础医学与临床,2013,33(7):854-858,5.基金项目
广西壮族自治区教育厅资助项目(200911MS187) （200911MS187）
