基础医学与临床2013，Vol.33Issue(7)：864-867,4.
磷酸肌酸减少大鼠缺血再灌注心肌细胞凋亡及自噬泡的数量
Creatine phosphate decreases the apoptosis and autophagy in myocardial ischemia/reperfusion rat heart
摘要
Abstract
Objective To investigate the role of Creatine phosphate in myocardial ischemia-reperfusion (I/R) injury of rat heart.Methods Twenty-four male SD rats weighing 200 ～250 g,were randomly divided into a Sham group; a I/R group and a phosphocreative (CP)group.CP group were taken the intravenous administration of 4 mg/kg creatine phosphate sodium before the reperfusion.TUNEL method was used to detect apoptosis of cardiomyocytes.The formation of autophagosomes was observed by electron microscopy.Expression of LC3-Ⅱ was measured by the Western blot.Results Comparing with sham group,I/R aggravated injury of mitochondria,and increased autophagic vacuoles (AVs) (P ＜ 0.01) and apoptosis of cardiomyocytes (P ＜ 0.01).However,CP group alleviated injury of mitochondria and reduced autophagic vacuoles(P ＜ 0.05) and apoptosis of cardiomyocytes(P ＜0.05) comparing to I/R group.LC3-Ⅱ formation,an autophagy marker,was down-regulated in the CP group (P ＜ 0.01),which was less increased than I/R-injured rats (P ＜ 0.05).Conclusions Creatine phosphate inhibits apoptosis and excessive autophagy to diminish the cell death induced by the myocardial I/R injury.关键词
自噬/缺血再灌注损伤/磷酸肌酸/凋亡Key words
autophagy/ ischemia-reperfusion injury/ creatine phosphate/ apoptosis分类
医药卫生引用本文复制引用
刘艺,徐卫娟,柯丽,李云桥,彭雯..磷酸肌酸减少大鼠缺血再灌注心肌细胞凋亡及自噬泡的数量[J].基础医学与临床,2013,33(7):864-867,4.基金项目
湖北省自然科学基金(2011CDB382) （2011CDB382）
