基础医学与临床2013，Vol.33Issue(7)：888-891,4.
RNAi介导的pirin低表达降低了K562细胞的红系分化能力
RNA interference-mediated pirin low expression reduces the erythroid differentiation of K562 cells
摘要
Abstract
Objective This article is aimed at researching the influence of pirin knocking down on the differentiation and proliferation of K562 cells.Methods Techniques of shRNA interference was applied to generate stable cell strain of pirin low expression.Western blot was used to analyze the interference efficiency of pirin-shRNA.MTT and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in the presence of inducers.Results Western blot showed that the pirin knockdown stable cell strain had a lower expression of pirin as compared to the control group,MTT results showed that there was no difference between the pirin knockdown cell strain and the control group,but the hemoglobin synthesis had been reduced as compared to the control group.Conclusions Pirin knocking down does not affect the cell viability of K562 cells,but inhibit the erythroid differentiation of K562 cells.关键词
K562细胞/慢病毒/pirin/红系分化/白血病Key words
K562 cells/ Lentivirus/ pirin / erythroid differentiation / leukemia分类
医药卫生引用本文复制引用
毛群铨,王婕,曹玲玲,薛建有,戚武林,朱晓芳,张世馥..RNAi介导的pirin低表达降低了K562细胞的红系分化能力[J].基础医学与临床,2013,33(7):888-891,4.基金项目
浙江省生物医学开放基金(SWYX0902) （SWYX0902）
