解放军医学杂志2013，Vol.38Issue(6)：476-480,5.
微流控芯片上胰岛素样生长因子1和碱性成纤维细胞生长因子对兔关节软骨细胞增殖的影响
Effects of insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) on the proliferation of rabbit articular chondrocytes embedded in microfluidic chip
李元城 1张卫国 1秦建华 2林炳承2
作者信息
- 1. 116001辽宁大连 大连医科大学附属第一医院骨科
- 2. 116001 辽宁大连 中国科学院大连化学物理研究所
- 折叠
摘要
Abstract
Objective To investigate the effects of insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) on the proliferation of chondrocytes in a 3-dimensional (3D) culture environment of microfluidic chip.Method Rabbit articular chondrocytes embedded in microfluidic chip and 96-well plate were cultured with solitary and component concentrations of IGF-1 and bFGF for 2 weeks,and then the proliferation rates of chondrocytes were calculated and compared.Results The maximal proliferation effect of IGF-1 was found when the concentration reached 57.14ng/ml (up to 2.38-fold),and of bFGF at the concentration of 5.72ng/ml (up to 3.85-fold),while the maximal proliferation effect of combination of IGF-I+bFGF was found at the concentration of 85.71ng/ml IGF-1 plus 1.43ng/ml bFGF (up to 4.76-fold).There was no significant difference between the proliferation rate of chondrocytes cultured in microfluidic chip and 96-well plate.Conclusions A combination of IGF-1 plus bFGF in certain concentration may synergistically increase the proliferation of chondrocytes.Microfluidic chip could be used for efficient cartilage tissue engineering.关键词
软骨细胞/组织工程/微流控芯片/胰岛素样生长因子1/成纤维细胞生长因子2Key words
chondrocytes/tissue engineering/microfluidic chip/insulin-like growth factor i/fibroblast growth factor 2分类
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李元城,张卫国,秦建华,林炳承..微流控芯片上胰岛素样生长因子1和碱性成纤维细胞生长因子对兔关节软骨细胞增殖的影响[J].解放军医学杂志,2013,38(6):476-480,5.
