吉林农业大学学报2013，Vol.35Issue(3)：351-354,4.
神经生长因子对猪卵丘细胞扩散及卵母细胞体外成熟的影响
Effect of Nerve Growth Factor (NGF) on Pig Cumulus Cell Expansion and Oocyte in vitro Maturation
摘要
Abstract
The aim of this work is to assess the effect of nerve growth factor on pig cumulus expansion and in vitro maturation (IVM) of pig oocytes.The degree of cumulus expansion was assessed directly under light microscope while nuclear maturation was assessed in terms of orcein staining.The results showed that NGF could not stimulate expansion of cumulus cells.Compared with oocytes in control group,oocytes stimulated with NGF alone were more likely to stay at GV phase,although the percentage of oocytes that had reached metaphase Ⅱ phase was not significantly different.Nevertheless,oocytes stimulated with NGF combined with gonadotrophins (equine chorionic gonadotropin and human chorionic gonadotropin) were more likely to reach metaphase Ⅱ phase compared with those stimulated with gonadotrophins alone.关键词
神经生长因子/猪/卵母细胞体外成熟/卵丘细胞扩散Key words
NGF/ pig/ oocyte in vitro maturation/ cumulus expansion分类
农业科技引用本文复制引用
陆晨,李万宏,陈树雄,吕岩,周虚..神经生长因子对猪卵丘细胞扩散及卵母细胞体外成熟的影响[J].吉林农业大学学报,2013,35(3):351-354,4.基金项目
国家重点基础研究发展计划项目(2011CB944203) （2011CB944203）
