解剖学杂志2013，Vol.36Issue(3)：269-273,5.DOI:10.3969/j.issn.1001-1633.2013.03.001
受激活调节正常T细胞表达和分泌因子及其受体CCR1、CCR5在大鼠附睾的表达与定位
Expression of regulated upon activation normal T-cell expressed and secreted and its receptors CCR1 and CCR5 in rat epididymis
摘要
Abstract
Objective:To observe the expression and the cellular localization of regulated upon activation normal T-cell expressed and secreted (RANTES) as well as its receptors CCR1 and CCR5 in the rat epididymis.Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of RANTES and its receptors mRNA in rat epididymis.The presence of RANTES and its receptors protein was assayed by Western blotting.Paraffin-embedded sections were prepared from rat epididymis,and immunohistochemical staining was carried out to detect cellular location of RANTES and its receptors.Colocalization of RANTES,CCR1,and CCR5 in rat epididymis was detected by immunofluorescence.Results:RT-PCR analysis showed the specific expression of RANTES and its receptors in the segments of adult rat epididymis.The distinctive single band of RANTES protein and its receptors protein was identified in the segments of rat epididymis with Western blotting.The positive staining of RANTES and its receptors were mainly observed in the basal cells of the epididymal epithelium.The immunofluorescence of RANTES coincided with CCR1 and CCR5 in the basal cells of epididymis.Conclusion:The expressions of RANTES and its receptors CCR1 and CCR5 were found restricted in the basal cells of the rat epididymis.These results revealed that RANTES may play a role in the physiological function of the basal cells of rat epididymis through autocrine or/and paracrine way.关键词
受激活调节正常T细胞表达和分泌因子/CCR1/CCR5/附睾/基细胞/大鼠Key words
regulated upon activation normal T-cell expressed and secreted/ CCR1/ CCR5/ epididymis/ basal cell/ rat引用本文复制引用
冯潇,程胖,赵洁,肖岚,李臻..受激活调节正常T细胞表达和分泌因子及其受体CCR1、CCR5在大鼠附睾的表达与定位[J].解剖学杂志,2013,36(3):269-273,5.基金项目
国家自然科学基金(81170614) （81170614）
陕西省自然科学基金(2010JM4001) （2010JM4001）
