解剖学杂志2013，Vol.36Issue(3)：330-335,6.DOI:10.3969/j.issn.1001-1633.2013.03.016
脑室周围白质软化大鼠膜铁转运蛋白1的表达变化及意义
Expression and significance of ferroportin 1 in rat models of hypoxic-ischemic periventricular leukomalacia
摘要
Abstract
Objective..To explore the expression and significance of ferroportin 1 (FPN1) in rat models of hypoxic-ischemic periventricular leukomalacia (PVL).Methods:Newborn 4-day-old SD rats were randomly divided into a control group and a hypoxic-ischemic PVL group.The cortices,hippocampi and periventricular white matters of rat brains were separated at 1 d,3 d,1 week and 4 week after operation.The iron concentrations were measured by graphite stove atomic absorption spectrometry.The distributions of FPN1 protein were tested by immunofluorescent histochemical staining method.The expressions of FPN1 mRNA and protein were respectively detected by real-time PCR and Western blotting.Results:Compared with the control group,there was no significant increase in iron concentration of each brain region in the PVL group at 1 d and 3 d after operation.While the increase in iron concentration was most significant in the periventricular white matter,second in the cortex and least in the hippocampus at 1 week after operation.Iron concentration was slightly lower in the cortex and it was reduced to the level of the control group in the hippocarmpus,but it was still at a high level in the periventricular white matter at 4 week after operation.Compared with the control group,there was no significant difference in the expression of FPN1 mRNA and protein in all brain regions in the PVL group at 1 d and 3 d after operation.But the levels of FPN1 were significanfly lower than those in the control group at 1 week after operation.At 4 week after operation,FPN1 level increased slightly in the cortex and it was close to the level of the control group in the hippocampus,while it was also significantly lower in the periventricular white matter than that of the control.Conclusion:The iron concentration increases and the expression of FPN1 decreases in a time and space-dependent manner in the rat brain with hypoxic-ischemic PVL,suggesting that down-regulation of FPN1 may cause the elevation of iron concentration to promote the development of PVL in the 4-day-old SD rats after hypoxia-ischemia.关键词
膜铁转运蛋白1/脑室周围白质软化/缺氧缺血/大鼠Key words
ferroportin 1/ periventricular leukomalacia/ hypoxia-ischemia/ rat引用本文复制引用
林清,张更,林如英,林穗金,王玮..脑室周围白质软化大鼠膜铁转运蛋白1的表达变化及意义[J].解剖学杂志,2013,36(3):330-335,6.基金项目
福建医科大学苗圃科研基金(2010MP028) （2010MP028）
