江苏大学学报（医学版）2013，Vol.23Issue(1)：1-4,4.
胞内高迁移率族蛋白B1对高糖诱导的小鼠心脏成纤维细胞转化生长因子-β1表达的影响
Effect of HMGB1 on the expression of TGF-β1 of cadiac fibroblast induced by high glucose
潘皓 1韩尧辉 1张赢予 1毛俊倩 1周艳芳 1王好 1鲍永辉 1赵龙 1张国辉1
作者信息
- 1. 江苏大学附属人民医院心内科,江苏镇江212002
- 折叠
摘要
Abstract
Objective:To investigate the effect of HMGB1 on the expression of TGF-β1 of cadiac fibroblast induced by high glucose.Methods:The cardiac fibroblasts were isolated from 3 w C57/BL6 mice.The identification of cardiac fibroblasts was evaluated by immunocytochemical staining with vimentin.The cardiac fibroblasts were treated by high glucose for different times(0,6,12,24,48 h).After transfected with siR-NA,the cardiac fibroblasts were treated by high glucose for 6h.The mRNA levels of HMGB1 and TGF-β1 of cardiac fibroblasts were determinated by RT-PCR.Results:The cultured cells were in accordance with characteristics of cardiac fibrolast.The mRNA levels of TGF-β1 of cardiac fibroblasts were inhibited by HMGB1 siRNA after treated by high glucose for 6 h.Conclusion:HMGB1 may contribute to modulate the expression of TGF-β1 of cardiac fibroblasts induced by high glucose.关键词
胞内高迁移率族蛋白B1/高糖/心脏成纤维细胞/转化生长因子-β1Key words
high mobility group box-1 / high glucose/ cardiac fibroblast/ transforming growth factor-β1分类
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潘皓,韩尧辉,张赢予,毛俊倩,周艳芳,王好,鲍永辉,赵龙,张国辉..胞内高迁移率族蛋白B1对高糖诱导的小鼠心脏成纤维细胞转化生长因子-β1表达的影响[J].江苏大学学报（医学版）,2013,23(1):1-4,4.
