江苏大学学报（医学版）2013，Vol.23Issue(1)：5-8,4.
α-硫辛酸对2型糖尿病大鼠肝脏氧化应激和内质网应激的影响
Effects of α-lipoic acid on liver oxidative stress and endoplasmic reticulum stress in type 2 diabetic rats
李静 1李瑞芳 2王国贤 1李云2
作者信息
- 1. 辽宁医学院药理学教研室,辽宁锦州121001
- 2. 邯郸市中医院脑外科,河北邯郸056001
- 折叠
摘要
Abstract
Objective:To investigate the protective effects of α-lipoic acid(A-LA) on liver in type 2 diabetic rats;the JNK and p-JNK protein expression related to ERS of liver in 2 diabetic rat.Method:Type 2 diabetes mellitus models were induced by high-fat diet and intraperitoneal injection of streptozotocin in fifty male Sprague Dawley rats.then divided into three groups:diabetic group,A-LA group and Vit E group.The groups except diabetic group treated with A-LA and VitE by intragastric for twelve weeks.The fasting glucose,blood lipid and serum liver function indices were measured after twelve weeks.Apoptosis was detected by TdT-mediated dUTP nick end labeling;Western blot analysis was employed to detect liver Phospho-c-Jun/JNK protein expression.Results:Compared with the control groups,diabetes model rats blood sugar and TG,TC,AST,ALT and MDA increased significantly (P ＜ 0.05),SOD and GSH-Px reduced significantly (P ＜ 0.05),the rate of cell apoptosis was increased.Protein expression of JNK didn't not change but phosphorylated JNK were significantly up (P ＜ 0.05).Compared with the diabetic group,A-LA groups above all indexes had been improved,the activities of SOD and GSH-Px were improved ; the rate of cell apoptosis was reduced,protein expression of p-JNK was down.Vit E group blood sugar was not changed,but the SOD and GSH-Px increased significantly.Conclusion:Alpha lipoic acid has a protective effect on liver in diabetic rats,the mechanism may be related to the inhibition of oxidative stress and endoplasmic reticulum stress关键词
2型糖尿病/α-硫辛酸/氧化应激/内质网应激Key words
type 2 diabetes mellitus/ α-lipoic acid/ oxidative stress/ endoplasmic reticulum stress分类
医药卫生引用本文复制引用
李静,李瑞芳,王国贤,李云..α-硫辛酸对2型糖尿病大鼠肝脏氧化应激和内质网应激的影响[J].江苏大学学报（医学版）,2013,23(1):5-8,4.
