江苏大学学报（医学版）2013，Vol.23Issue(1)：34-38,5.
非小细胞肺癌患者外周血中生存素和血管内皮细胞生长因子的表达及临床意义
Clinical significance of mRNA expression of survivin and vascular endothelial growth factor in peripheral blood from patients with non-small cell lung cancer
唐兴萍 1李坚 1汪毅2
作者信息
- 1. 江苏大学附属医院呼吸科,江苏镇江212001
- 2. 江苏大学附属医院中心实验室,江苏镇江212001
- 折叠
摘要
Abstract
Objective:To evaluate the clinical significance of mRNA expression of survivin and vascular endothelial growth factor (VEGF) in the peripheral blood from patients with non-small cell lung cancer (NSCLC).Methods:Real-time fluorescence quantitation polymerase chain reaction (RT-PCR) was used to detect mRNA expression of survivin and VEGF in peripheral blood samples from 42 patients with NSCLC and 20 patients with benign lung disease.Results:The mRNA expression of survivin and VEGF in NSCLC patients were significantly higher than those in benign lung disease patients (P =0.006 ; P =0.001,respectively).The positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with NSCLC were 59.5 ％ (25/42) and 57.1％ (24/42),respectively.Combined with the two mRNA expression made the positive rate raise to 76.2％,while the positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with benign lung disease were 5％ (1/20) and 0％,respectively,which were markedly lower than those of patients with NSCLC (both P ＜ 0.001).For patients with NSCLC,the tumor TNM stage was significantly correlated with mRNA expression of survivin and VEGF (P ＜ 0.001 ; P =0.008,respectively),whereas there were no correlation between mRNA expression of survivin and VEGF and other pathologic characteristics.Conclusion:The mRNA expression of survivin and VEGF in peripheral blood could be used as the tnolecular markers for diagnosis of NSCLC ; Combined with the two mR-NA expression in peripheral blood could raise the detection rate of cancer cell in peripheral blood.关键词
荧光定量PCR/非小细胞肺癌/生存素/血管内皮细胞生长因子Key words
fluorescence quantitation PCR/ non-small cell lung cancer/ survivin/ vascular endothelial growth factor分类
医药卫生引用本文复制引用
唐兴萍,李坚,汪毅..非小细胞肺癌患者外周血中生存素和血管内皮细胞生长因子的表达及临床意义[J].江苏大学学报（医学版）,2013,23(1):34-38,5.
