江苏大学学报（医学版）2013，Vol.23Issue(3)：191-196,6.
纳米银抗3型副流感病毒作用及机制研究
Potential mechanism and inhibitory effects of silver nanoparticles on parainfluenza virus type 3
摘要
Abstract
Objective:To explore the inhibitory effects and the potential mechanism of silver nanoparticles against parainfluenza virus type 3 (PIV3).Methods:Cell culture,MTT test assay and immune fluorescence assay were applied to evaluate the anti-PIV3 activities of silver nanoparticles in preventing,treating and directly inactivating.The neuraminidase activity inhibition test was used to study the inhibitory effect of silver nanoparticles on PIV3 neuraminidase activity; destructive effect of silver nanoparticles on PIV3 was observed by transmission electron microscope (TEM) ; murine experiment was performed to observe the pathological changes of mouse lung tissues in vivo.Results:The survival rates of canine kidney cells(MDCK) were 93.05％,90.32％ and 94.81％,respectively,under three different administration (treatment to medicine group,preventive medication group,direct inactivating group) of silver nanoparticles by MTT assay,while the cell livability in control group was 25.50％,the differences were statistically significant (P ＜0.01).MDCK cells infected with PIV3 had more specific yellowish-green immunofluorescence compared with the cells treated with silver nanoparticles in virucidal,antiviral and inactivating ways.The inhibition rate of neuraminidase activity on silver nanoparticles treatment group was higher than 80％,while the PIV3 control group and the solvent group were lower than 20％.The results of TEM showed that silver nanoparticles had an obvious destructive effect on PIV3 in a time-dependence.Compared with the normal lung tissues,the pathological changes in the mice treated with the silver nanoparticles were similar or less severe,while the lung tissues from the virus model group presented with edema,inflammatory cell infiltrating and morphological structure disappearing.Conclusion:Silver nanoparticles had remarkably inhibitory effects on PIV3 and the possible mechanisms may be associated with the neuraminidase inhibition and virion damage by silver nanoparticles.关键词
纳米银/副流感病毒/抗病毒作用/作用机制Key words
silver nanoparticles / parainfluenza virus / inhibitory effect / mechanism分类
医药卫生引用本文复制引用
尹俭俭,李秀景,郑丛龙..纳米银抗3型副流感病毒作用及机制研究[J].江苏大学学报（医学版）,2013,23(3):191-196,6.基金项目
国家自然科学基金资助项目(30870681) （30870681）
