江苏大学学报（医学版）2013，Vol.23Issue(3)：197-200,206,5.
糖尿病心肌病小鼠心肌成纤维细胞miR-375表达及其对胶原蛋白Ⅰ合成的影响
Expression and effect of miR-375 on synthesis of collagen Ⅰ in mice with diabetic cardiomyopathy
摘要
Abstract
Objective:To investigate the effect of miR-375 on cardiac fibrosis in diabetic cardiomyopathy mice.Methods:Cardiac fibroblasts isolated from hearts of C57BL/6 mice were cultured in DMEM medium (low glucose concentration).The purity of cardiac fibroblasts were evaluated by immunohistochemical staining.The second and third generation cardiac fibroblasts were randomly divided into control group (5 mmol/L glucose)and high glucose group (25 mmol/L glucose).The cardiac fibroblasts of high glucose groups were treated by high glucose for different times (6,12,24 h).The different expression of miR-375 in cardiac fibroblasts was detected by real-time quantitative PCR.miR-375 inhibitor was transiently transfected into cardiac fibroblasts by lipofectamineTM 2000 reagent; transfection efficiency was detected through real-time quantitative PCR.The expression of collagen Ⅰ in cardiac fibroblasts was evaluated by immunohistochemical staining.Results:The purity of the second generation of cardiac fibroblasts was above 90％.During the time point 6 to 24 h cardiac fibroblasts treated by high glucose,the expression of miR-375 showed an increase tendency;in high glucose and miR-375 inhibitor treated group,the expression of collagen Ⅰ decreased significantly.Conclusion:MiR-375 might play an important role in diabetic myocardial fibrosis.关键词
MiR-375/糖尿病心肌病/心肌纤维化Key words
MiR-375/ diabetic cardiomyopathy/ fibrosis分类
医药卫生引用本文复制引用
黄燕,张赢予,王好,周艳芳,张国辉,芮涛..糖尿病心肌病小鼠心肌成纤维细胞miR-375表达及其对胶原蛋白Ⅰ合成的影响[J].江苏大学学报（医学版）,2013,23(3):197-200,206,5.基金项目
国家自然科学基金资助项目(批准号:81170210) （批准号:81170210）
