江苏大学学报（医学版）2013，Vol.23Issue(3)：201-206,6.
炎症环境下巨噬细胞对小鼠骨髓间质干细胞迁移能力的影响
Effect of macrophages on the migration of mouse bone marrow mesenchymal stem cells in inflammation
摘要
Abstract
Objective:To investigate the effect of macrophage cell line RAW264.7 on the migration and expression of TGF-β1,IL-6 and MCP-1 gene level of mouse bone marrow derived mesenchymal stem cells (MSCs) under the stimulation of lipopolysaccharide(LPS).Methods:Adherent method was used to isolate mouse bone marrow mesenchymal stem cells.MSCs were co-cultured with RAW264.7 through transwell assay.Co-culture experiments were divided into four groups:culture medium control,RAW264.7 cells,LPS and RAW264.7 cells stimulated by LPS.Cell transwell migration assay were performed to detect the number of migrated MSCs under the four different conditions.At the same time,qRT-PCR was performed for the quantification of TGF-β1,IL-6 and MCP-1genes.Results:RAW264.7 expressed high level of TNF-α,IL-6,IL-1 β and MCP-1 gene by LPS stimulation.In the presence of RAW264.7,the migrated MSCs numbers were higher than controls (P ＜ 0.01),while RAW264.7 cells remarkably increased the migratory ability of MSCs under the stimulation of LPS (P ＜ 0.01).Meanwhile,the expression of TGF-β1,IL-6 and MCP-1 genes were enhanced by co-culture with RAW264.7 in the presence of LPS (P ＜ 0.05).Conclusion:Macrophage cell line RAW264.7 enhanced the migratory ability and exchanged the cytokine expression of MSCs under the stimulation of LPS,which provided mechanistic insights into the recruitment of MSCs in inflammation.关键词
骨髓间质干细胞/巨噬细胞/细胞迁移/细胞因子/炎症Key words
mesenchymal stem cell / macrophage / migration / cytokine / inflammation分类
医药卫生引用本文复制引用
高硕,蔡梦洁,毛飞,杨婷婷,张洁,张玲,赵露露,坎吉克孜·阿不来提,钱晖..炎症环境下巨噬细胞对小鼠骨髓间质干细胞迁移能力的影响[J].江苏大学学报（医学版）,2013,23(3):201-206,6.基金项目
国家自然科学基金重大研究计划资助项目(91129718) （91129718）
国家自然科学基金资助项目(81000181) （81000181）
江苏省高校自然科学基金资助项目(10KJB310002) （10KJB310002）
江苏大学高级人才启动基金资助项目(10JDG094) （10JDG094）
江苏大学大学生科研立项课题(11A387) （11A387）
