江苏大学学报（医学版）2013，Vol.23Issue(3)：212-215,4.
乌头碱对胎鼠中脑多巴胺能神经元细胞的神经毒性作用
Neurotoxic effect of aconitine based on the midbrain dopaminergic neurons cell culture
摘要
Abstract
Objective:To observe toxicological effects of aconitine on midbrain dopaminergic neurons and to understand and prevent neurotoxic side effects from aconitine and its derivatives.Methods:Fetal rat brain was taken out from the 14 days pregnant mice and dopaminergic neurons were isolated to culture in 5％ CO2,37 ℃ and 100％ humidity conditions.Different concentrations of aconitine (0.1,0.5,1,5,10,50 and 100 μmol/L) were added on 10th day in vitro and treated for 48 hours.Immunohistochemical staining was done on 12th day and the number of cells,dendritic length and the number of neuron axons were selected as observed indicators,and to make the analysis.Results:Forty-eight hours later,different concentrations of aconitine(0.5-100 μmol/L) had strong inhibition on growth of neuron cells,which showed a significant concentration-dependent neurotoxicity.While the high concentrations of aconitine (10 ～ 100 μmol/L) had stronger influence on neuron cells than the low concentrations of aconitine (0.5 ～5 μmol/L).Conclusion:The neurotoxicity effects of aconitine on midbrain dopaminergic neurons in vitro experiments were confirmed for the first time.关键词
乌头碱/多巴胺能神经元/神经毒性/细胞培养Key words
aconitine / dopaminergic neurons / neurotoxicity / cell culture分类
医药卫生引用本文复制引用
严妍,杨茂,Wolf-dieter Rausch,王喜军..乌头碱对胎鼠中脑多巴胺能神经元细胞的神经毒性作用[J].江苏大学学报（医学版）,2013,23(3):212-215,4.基金项目
国家中医药管理局基金资助项目(200807014) （200807014）
Austrian Ministry for Research GZ(BMWF-402.088/8-Ⅱ/6b/2008) （BMWF-402.088/8-Ⅱ/6b/2008）
