江苏大学学报（医学版）2013，Vol.23Issue(3)：220-223,4.
基于口服免疫耐受原理的人肿瘤小鼠荷瘤模型的建立
Establishment of a mouse model of human tumor based on principle of oral tolerance
摘要
Abstract
Objective:To establish a mouse model of human tumor-specific immune tolerance based on principle of oral tolerance.Methods:Ninety mice were randomly divided into three groups:blank control group,irrelevant tumor control group and experimental tumor model group,thirty in each group.The mice of three groups were received intragastric administration of normal saline,human ovarian cancer cell line 3AO and human hepatoma cell line HepG2 cells for 7 days,respectively.Then,all mice received subcutaneous injection of HepG2 cells in the back skin.The time course of the tumor incidence were recorded and the size of tumor were determined by slide caliper.The histological examination of sections of tumor tissues were evaluated by optical microscope.Results:HepG2 cells in mice from experimental tumor model group survived and developed into tumor after subcutaneous injection of HepG2 cells.The tumor incidence was 90％ (27/30).There was no tumor formation in the mice of blank control group and irrelevant tumor control group.Obviously pathologic changes were found in tumor tissue sections with HE staining,while some debris of death tumor cells were found in mice from control group and irrelevant tumor control group.Conclusion:The mouse model of human tumor was established successfully by intragastric administration of HepG2 cells through induction of specific immune tolerance.关键词
口服免疫耐受/小鼠/肿瘤模型/人肝癌HepG2细胞系Key words
oral immune tolerance/ mouse/ tumor models/ human hepatoma cell line HepG2 cells分类
医药卫生引用本文复制引用
刘霞,成静,范兴文,周小兵,周丹,吴卫疆,华晔,邵启祥..基于口服免疫耐受原理的人肿瘤小鼠荷瘤模型的建立[J].江苏大学学报（医学版）,2013,23(3):220-223,4.基金项目
国家自然科学基金资助项目(81273202) （81273202）
江苏省自然科学基金资助项目(BK2008231) （BK2008231）
