江西农业大学学报2013，Vol.35Issue(3)：502-506,5.
SSR标记在杂交旱稻新组合纯度鉴定中的应用
Application of SSR Molecular Marker to Purity Identification of New Hybrid Upland Rice
摘要
Abstract
600 SSR primers distributed in 12 rice chromosomes were used to analyse their complementary pattern in this study.The purity of hybrid rice seeds was identified through identifying twenty-one new combinations of hybrid upland rice and their seventeen parents and by using the Tween method to extract DNA and utilizing the molecular marks such as RM 3,RM 304 and RM 475.The results were as follows:the purity of new 1A/hanhui 3 and 603A/hanhui 25 was 98.9％,so their purity was the best; meanwhile the purity of 11 A/hanhui 29 was 87.5％,its purity was the worst among all the combinations;and the purity of other combinations was about 95 ％.关键词
SSR标记/杂交旱稻/纯度鉴定Key words
SSR marker/ hybrid upland rice / purity identification分类
农业科技引用本文复制引用
张琳,杜鹃,齐丽霞,胡颂平,魏志刚,杨莉,张安宁,刘国兰..SSR标记在杂交旱稻新组合纯度鉴定中的应用[J].江西农业大学学报,2013,35(3):502-506,5.基金项目
国家自然科学基金项目(31260314)、江西省自然科学基金项目(2010GZN0038)、江西省教育厅重点项目(GJJ11018)和江西农大人事基金项目(3136) （31260314）
