昆虫学报2013，Vol.56Issue(5)：494-498,5.
氟化物对家蚕耐氟和氟化物敏感品种幼虫中肠羧酸酯酶及全酯酶活性的影响
Influence of fluoride on activities of carboxylesterases and esterases in the larval midgut of the fluoride-resistant and susceptible strains of Bombyx mori
摘要
Abstract
To explore the metabolic pathway of NaF in the silkworm,Bombyx mori,the 5th instar larvae of the fluoride-resistant silkworm strain (T6) and the fluoride-susceptible strain (734) were fed on mulberry leaves soaked in 50,100,200 and 400 mg/kg NaF solutions,respectively,for 1-7 d,and the activities of carboxylesterases (CarE) and esterases in the midgut were examined.The results showed that the CarE activity in strain 734 was 1.21-1.98-fold as high as that in the control group,while the CarE activity in strain T6 was 0.72-1.10-fold as high as that in the control group.In both strains treated with NaF,the change trend of the esterase activity was similar with the control group within 7 d,and the esterase activity in the two strains was also similar.When the two silkworm strains were treated by NaF at the same concentration,their esterase activities on different days after treatment were significantly different (P ＜ 0.05).It is inferred that fluoride can increase the CarE activity in the midgut of the fluoride-susceptible strain of the silkworm,and inhibit the CarE activity in the midgut of the fluoride-resistant strain,but it has little effect on the esterase activity in the midgut of both strains of the silkworm.关键词
家蚕/氟化物/中肠/羧酸酯酶/全酯酶/酶活性Key words
Bombyx mori/ fluoride / midgut / carboxylesterase / esterase / enzyme activity分类
生物科学引用本文复制引用
米智,阮成龙,李姣蓉,付巧娟,武婧洁,SENDEGEYA Parfait,朱勇..氟化物对家蚕耐氟和氟化物敏感品种幼虫中肠羧酸酯酶及全酯酶活性的影响[J].昆虫学报,2013,56(5):494-498,5.基金项目
重庆市重大攻关项目(CSTC,2009AA1024) （CSTC,2009AA1024）
