昆虫学报2013，Vol.56Issue(5)：554-560,7.
海南岛中华蜜蜂遗传多样性的微卫星DNA分析
Microsatellite DNA analysis of genetic diversity of Apis cerana cerana in Hainan Island, southern China
摘要
Abstract
To assess the genetic diversity and genetic structure of Apis cerana cerana populations in Hainan Island,southern China and their relationship with the mainland populations,627 individual workers from 627 colonies in 11 localities of Hainan Island and 102 individuals from 102 colonies in two localities of mainland China were genotyped using 10 microsatellite DNA loci.The results showed a high level of allelic diversity with 5-17 alleles across all the loci and an average of 4.5-7.0 alleles per locus across Hainan Island populations.The mean expected heterozygosity (He) values across all samples ranged from 0.59 to 0.65.The populations from Hainan Island showed similar allelic structure pattern at ten loci,but Wenchang and Tunchang populations shared a unique allelic pattern at AT101.The FST between Hainan Island populations and adjacent mainland ones ranged from 0.06 to 0.13.The FST of Wenchang and Tunchang compared to other nine island populations ranged from 0.06 to 0.12,which was larger than that between the nine island populations (FST =0-0.05).The results showed Hainan Island A.c.cerana populations diverged from the neighboring mainland ones.Both Wenchang and Tunchang populations showed a higher level of differentiation from other nine island populations among which slight differentiation occurred.This study provides a theoretical support for efficiently preserving Hainan indigenous A.c.cerana as a genetic resource for future utilization.关键词
中华蜜蜂/遗传多样性/微卫星DNA/等位基因/杂合度/海南岛Key words
Apis cerana cerana / genetic diversity / microsatellite DNA / alleles / heterozygosity /Hainan Island分类
生物科学引用本文复制引用
徐新建,周姝婧,朱翔杰,周冰峰..海南岛中华蜜蜂遗传多样性的微卫星DNA分析[J].昆虫学报,2013,56(5):554-560,7.基金项目
国家现代蜂产业技术体系建设专项资金(CARS-45-KXJ11) （CARS-45-KXJ11）
农业部热带农林有害生物入侵检测与控制重点开放实验室开放课题基金(MACKL1002) （MACKL1002）
