昆明医科大学学报2013，Vol.34Issue(5)：29-33,5.
颅咽管瘤中RARα及PPARβ/δ表达与RA作用的相关性
Correlation between Expression of RAR and PPARβ/δ and Effect of Retinoic Acid in Craniopharyngioma Cells
摘要
Abstract
Objective To detect the expression of Retinoic acid receptor alpha (RAR α) and peroxisome proliferator-activated receptor β / δ (PPAR β / δ) in craniopharyngioma cells,analyze the correlation between the expression and effect of retinoic acid (RA),and investigate the molecular mechanism of RA targeted therapy for craniopharyngioma.Methods The expression of RAR α and PPAR β/δ in 31 cases of craniopharyngioma cells in vitro was quantified by reverse transcription-PCR.The inhibition rates of RA in craniopharyngioma cells with different expression levels of RARα and PPAR β / δ were detected by using MTT assay.And the correlation between expression and effect was analyzed.Results The RT-PCR results showed that the expression of PPAR β / δ and RARα mRNA in craniopharyngioma cells was different.31 cases of craniopharyngioma cells in primary culture were divided into three groups according the expression differences of nuclear receptor:PPAR β / δ ＞ RAR α,RARα ＞PPAR β / δ and RAR α ＞＞ PPAR β / δ.MTT results showed that the inhibition rate of RAR α ＞＞ PPAR ββ / δ group was significantly higher than the other groups under the same condition,with statistical significance (P ＜0.01).Conclusion The expression of PPAR β / δ and RAR α can be used as effective index to evaluate the effect of RA in craniopharyngioma.The craniopharyngioma with low-expression PPAR β/δ was more sensitive to RA.Targeting higher RARα or targeting lower PPAR β / δ is beneficial to the treatment of craniopharyngioma.关键词
过氧化物酶体增殖物活化受体/β/δ核维甲酸受体/颅咽管瘤Key words
Peroxisome proliferator-activated receptor β / δ (PPAR β / δ) / Retinoic acid receptor (RAR) / Craniopharyngioma分类
医药卫生引用本文复制引用
白刚,罗林,王行桥,倪炜,左频,袁红平,牛华涛,范耀东..颅咽管瘤中RARα及PPARβ/δ表达与RA作用的相关性[J].昆明医科大学学报,2013,34(5):29-33,5.基金项目
云南省科技厅专项研究基金资助项目(2008CD038) （2008CD038）
