昆明医科大学学报2013，Vol.34Issue(5)：69-73,5.
姜黄素对PC-3前列腺癌细胞株生长抑制的体外实验研究
Growth Inhibition Effect of Curcumin on Prostate Cancer PC-3 Cells in Vitro
摘要
Abstract
Objective To investigate the growth inhibition effect and mechanism of curcumin on prostate cancer PC-3 cells.Methods The growth inhibition effect of curcumin on PC-3 cells was determined using 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay.Apoptosis rate was detected by Flow cytometry (FCM) The expression of fatty acid synthase (FASN) mRNA and BCL2-associated X protein (BAX) mRNA in response to curcumin was inspected by reverse transcription-polymerase chain reaction (RT-PCR).Results After 24 and 48 hours,the growth of PC-3 cells was suppressed obviously by curcumin in a time-and dose-dependent manner in vitro.Compared with control group,the inhibition rate of each experimental group was statistically significant (P ＜ 0.01).After PC-3 cells were treated with curcumin for 24 hours,apoptosis rate increased with the increase of the concentrations,and achieved maximum when cells were treated at 40 μmol/L.Compared with control group,the apoptosis rate of each experimental group was statistically significant (P ＜0.01).After PC-3 cells were treated with different concentrations of curcumin,the expression of FASN mRNA was significant reduced with the increase of the concentrations.However,the expression of BAX mRNA was significant increased with the increase of the concentrations.Gonclusion Curcumin could significantly inhibit the growth of prostate cancer PC-3 cells,suppress the expression of FASN mRNA,and increase the expression of BAX mRNA.Inducing PC-3 cells apoptosis may be one of the mechanisms of curcumin.关键词
脂肪酸合成酶/姜黄素/前列腺癌Key words
FASN/ Curcumin/ Prostate cancer分类
医药卫生引用本文复制引用
刘孝东,孔祥明,张建华,陈剑珩,申吉泓..姜黄素对PC-3前列腺癌细胞株生长抑制的体外实验研究[J].昆明医科大学学报,2013,34(5):69-73,5.基金项目
云南省校联合基金资助项目(2011FB173) （2011FB173）
