临床神经病学杂志2013，Vol.26Issue(3)：202-205,4.
神经生长因子联合康复训练对脑梗死大鼠神经行为学及脑源性神经营养因子、Bax表达的影响
Effect of nerve growth factor combined with rehabilitation training on the neurological behavior and expression of brain-derived neurotrophic factor, Bax in cerebral infarction rats
王魁花 1白蓉 2胡慧华 1史银玲 2张由建2
作者信息
- 1. 450052 郑州大学第五附属医院康复科
- 2. 郑州大学第一附属医院神经内科
- 折叠
摘要
Abstract
Objective To investigate the effect of nerve growth factor (NGF) combined with rehabilitation training on the neurological behavior and expression of brain-derived neurotrophic factor (BDNF),pro apoptotic protein Bax in cerebral infarction rats.Methods Seventy two SD rats were randomly divided into control group,NGF group,rehabilitation training group and NGF combined with rehabilitation training group (combined treatment group).Each group was divided into 7 d,14 d,21 d after cerebral infarction subgroups.The cerebral infarction rat model was established with suture method.NGF group was intraperitoneally injected NGF 20 μg/(kg · d) ; rehabilitation training group was training by balance beam,rolling-bar and net screen 72 h after establish model; combined treatment group was given NGF and rehabilitation training.The neurological behavior scores were evaluated,the BDNF,Bax and their mRNA expression of brain tissue were detected with immunohistochemical staining and RT-PCR at the corresponding time points in each group respectively.Results Compared with control group,the neurological behavior scores in each time point subgroups of NGF group,rehabilitation training group and combined treatment group were improved ; and the expressions of BDNF and BDNF mRNA were significantly increased,the expressions of Bax and Bax mRNA were significantly decreased (all P ＜ 0.05).Compared with combined treatment group,the neurological behavior scores in each time point subgroups of NGF group and rehabilitation training group were significantly increased,and the expressions BDNF and BDNF mRNA were significantly decreased,the expressions of Bax and Bax mRNA were significantly increased (all P ＜ 0.05).Conclusion The NGF combined with rehabilitation training can up-regulate the expression of BDNF and down-regulate the expression of Bax,and significantly improve the nerve function recovery in rats with cerebral infarction.关键词
神经生长因子/康复训练/脑梗死/脑源性神经营养因子/BaxKey words
nerve growth factor/ rehabilitation training/ cerebral infarction/ brain-derived neurotrophic factor/ Bax分类
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王魁花,白蓉,胡慧华,史银玲,张由建..神经生长因子联合康复训练对脑梗死大鼠神经行为学及脑源性神经营养因子、Bax表达的影响[J].临床神经病学杂志,2013,26(3):202-205,4.
