临床神经病学杂志2013，Vol.26Issue(3)：206-209,4.
5-羟色胺1A受体激动剂8-OH-DPAT对癫痫合并抑郁大鼠的干预作用及其机制研究
Study of intervention effect and its mechanism of 5-hydroxytryptamine 1A receptor agonist 8-OH-DPAT on epileptic rats with depression
摘要
Abstract
Objective To study intervention effect and its mechanism of 5-hydroxytryptamine 1A (5-HT1A)receptor agonist 8-OH-DPAT on epileptic rats with depression.Methods Eight rats were randomly selected from 160 adult SD rats as normal control (NC) group,the other rats were induced epilepsy with pilocarpine.After 25 d,32 epileptic rats with depression were selected by the body weight and food intake measurements and open field test,and were randomly divided into model group,carbamazepine (CBZ) group,CBZ + 8-OH-DPAT low dose group(8-OH-DPAT low dose group) and CBZ + 8-OH-DPAT high dose group(8-OH-DPAT high dose group).The rats in each group were respectively treated with normal saline,CBZ 100 mg/kg,CBZ +8-OH-DPAT 0.1 mg/(kg · d),CBZ +8-OH-DPAT 1 mg/(kg · d).Racine classification,body weight and food intake and open field test were measured 7 d after continuous coursetreatment.The expression of nerve growth factor(NGF) mRNA was measured with fluorescence quantitative PCR,mossy fiber sprouting (MFS)was observed with Timm staining.Results Compared with the NC group after treatment,weights,food intake in model group,CBZ group,8-OH-DPAT low and high dose groups were significantly decreased ; the expression of the NGF mRNA and timm scores were significantly increased (all P ＜0.05).Compared with model group,weight,food intake,open-field test scores in CBZ group,8-OH-DPAT low and high dose groups were remarkably increased; Racine classification,the expression of NGF mRNA and Timm scores were remarkably decreased (all P ＜ 0.05).The above changes were more obvious in the 8-OH-DPAT high dose group(all P ＜ 0.05).Conclusion High doses of 5-HT1A receptor agonist 8-OH-DPAT can inhibit MFS and NGF expression of hippocampal dentate gyms in epileptic rats with depression,to promote hippocampal neural remodeling.That may be one of the molecular mechanisms of antidepressants and antiepileptic by 5-HT1A receptors agonist.关键词
癫痫/抑郁/5-羟色胺1A受体激动剂/海马/苔藓纤维发芽/神经生长因子Key words
epilepsy / depression/ 5-hydroxytryptamine 1A receptor agonist / hippocampal / mossy fiber sprouting/ nerve growth factor/分类
医药卫生引用本文复制引用
李琼,孙美珍,杨萍..5-羟色胺1A受体激动剂8-OH-DPAT对癫痫合并抑郁大鼠的干预作用及其机制研究[J].临床神经病学杂志,2013,26(3):206-209,4.基金项目
山西省归国人员基金(2008-52) （2008-52）
