林业科学2013，Vol.49Issue(5)：43-53,11.DOI:10.11707/j.1001-7488.20130507
应用nanoLC-MS/MS分析毛白杨次生维管系统的蛋白质表达谱
Proteome Profile in Secondary Vascular System of Populus tomentosa by nanoLC-MS/MS
摘要
Abstract
Abstract:As the uppermost carbon pool and important renewable source,wood has dual functions in both responding to the global climate change and meeting the resource demands of human society,thereby it is essential to understand the molecular mechanism of wood formation.In this study,a 1DE-based nanoLC-MS/MS method was introduced to analyze the proteome in the secondary vascular system of Populus tomentosa,and the identified proteins included:14 proteins associated cell division; 7 proteins related to transcription factors; 22 proteins associated with cytoskeleton; 31 proteins associated with signal transduction;and 14 proteins associated with cell wall formation.The corresponding genes of these proteins participate in regulating cambial activity.The accomplishment of this proteome analysis laid a foundation for further understanding the molecular mechanism of wood formation.关键词
液相色谱-串联质谱/蛋白质表达谱/次生维管系统/毛白杨Key words
LC-MS/MS/ proteome profile/ secondary vascular system/ Populus tomentosa分类
农业科技引用本文复制引用
蒋淑磊,陈加飞,赵树堂,卢孟柱..应用nanoLC-MS/MS分析毛白杨次生维管系统的蛋白质表达谱[J].林业科学,2013,49(5):43-53,11.基金项目
国家自然科学基金重点项目(31030018). （31030018）
