南京医科大学学报（自然科学版）2013，Vol.33Issue(6)：715-721,7.DOI:10.7655/NYDXBNS20130601
筛选调控Sonic Hedgehog信号转导的泛素连接酶
Identification of E3 ligases that regulate Sonic Hedgehog signaling transduction
摘要
Abstract
Objeetive:Screening for HECT E3 ligases that can regulate Sonic Hedgehog (Shh) signaling pathway.Methods:In the first round of screening,siRNAs of the HECT E3 ligases were tested for Shh pathway transduction by GliBS-luciferase assay.And in the second round,these siRNAs were tested for the localization of Patched1-GFP in primary cilia by immunofluorescence staining.Results:After two rounds of screening,5 HECT E3 ligases were identified to regulate Shh pathway,which are Smurf1,Smurf2,Ube3c,Wwp1 and Wwp2.Conclusion:A screening system of new regulators of Shh signaling was setup,and 5 HECT E3 ligases were found from our preliminary screening.关键词
Sonic Hedgehog信号通路/HECT E3泛素连接酶/Patched1/原纤毛Key words
Sonic Hedgehog signaling/ HECT E3 ligases/ Patched1 / primary cilia分类
生物科学引用本文复制引用
汤颖,乐珅,程雁..筛选调控Sonic Hedgehog信号转导的泛素连接酶[J].南京医科大学学报（自然科学版）,2013,33(6):715-721,7.基金项目
教育部博士学科点基金(20103234110003) （20103234110003）
国家自然科学基金国际(地区)合作与交流项目(81261120386) （地区）
国家自然科学基金青年基金项目(81101497) （81101497）
