内科理论与实践2013，Vol.8Issue(3)：187-191,5.DOI:10.3969/j.issn.1673-6087.2013.03.008
青蒿素衍生物SM1044对急性髓系白血病细胞株HL60作用的研究
Effects of artemisinin derivative SM1044 on acute myeloid leukemia cell line HL60
摘要
Abstract
Objective To investigate the proliferation inhibition and apoptosis-inducing effects and its potential mechanisms of artemisinin derivative SM1044 on HL60 cells (acute myeloid leukemia cell line).Methods Cell counting kit8 (CCK-8)assay was performed to detect the proliferation inhibition induced by SM1044 on HL60 cells.Cell apoptosis and mitochondria membrane potential were assessed with flow cytometry.Expressions of apoptosis related proteins caspase-3,caspase-8,caspase-9 and poly ADP-ribose polymerase (PARP) were determined by Western blot.Results SM1044 displayed significant anti-proliferative effect on HL60 cells,with 50％ inhibiting concentration (IC50) value of 0.053 μmol/L,and its anti-proliferative effect was better than other artemisinin derivatives.Furthermore,it induced apoptosis and reduced mitochondria membrane potential of HL60 cells in a dose-dependent manner.As the HL60 cells were treated with the different concentrations of SM1044 (0,0.05,0.1 and 1 μ mol/L) for 24 h,the cell apoptosis rates were 3.15％,16.75％,32.53％ and 49.33％ respectively(r=0.947,P＜0.05),and the rates of mitochondrial transmembrane potential loss were 10.3％,26.1％,32.7％ and 51.25 respectively (r=0.963,P＜0.05).In addition,SM1044 could induce the activation of caspase-8 and caspase-9,then activated caspase-3 and finally cleaved PARP.Conclusions SM1044 efficiently inhibited proliferation and induced apoptosis of HL60 cells,which could be caused by both the extrinsic and intrinsic apoptotic pathways.关键词
青蒿素衍生物/急性髓系白血病/HL60细胞/细胞增殖/细胞凋亡Key words
Artemisinin derivative/Acute myeloid leukemia/HL60 cell/Cell proliferation/Cell apoptosis分类
医药卫生引用本文复制引用
彭宇,宋志群,赵玲玲,王月英,王瑾,李英,糜坚青..青蒿素衍生物SM1044对急性髓系白血病细胞株HL60作用的研究[J].内科理论与实践,2013,8(3):187-191,5.基金项目
973国家重大基础研究发展计划(项目编号:2010CB529203) （项目编号:2010CB529203）
上海市慈善癌症研究中心慈善慢跑课题 （）
