农业环境科学学报2013，Vol.32Issue(6)：1122-1127,6.DOI:10.11654/jaes.2013.06.006
纳米氧化锌和二氧化钛对斜生栅藻的毒性效应
Ecotoxicological Effects of ZnO and TiO2 Nanoparticles on Microalgae Scenedesmus oblignus
摘要
Abstract
With the mass production and widespread use of nanomaterials,the potential for their release into the environment is increasing.The potential risks and ecological effect to human health and ecological environment has become a new field of scientific research.In this study the impact of nano-ZnO and nano-TiO2 on aquatic ecosystems has been investigated using microalage Scenedesmus oblignus as a model.Microalgae S.oblignus were exposed to nano-ZnO and nano-TiO2 at different doses(0.01,0.1,0.5,1,5,50 mg·L-1 and 1,5,10,50,100,200 mg· L-1) for 96 h.Response of the growth,photosynthetic pigments and antioxidant enzyme activities were selected as assessment indicators.The results indicated that the effect of nano-ZnO within the concentrations of 0.01,0.05,1 mg· L-1 on the growth of S.oblignus showed a significant improvement(P＜0.05) but no significant difference on protein content compared with control.However,when the concentration was increased to 5 mg· L-1,nano-ZnO started to bring an evident stress to the growth and protein content,and high concentrations of 50 mg· L-1 significantly inhibited the growth and protein content,while the inhibitation of photosynthetic pigments,and superoxide dismutase (SOD) and peroxidase (POD) activities was observed in lower concentrations.Similarly,dose-effects of nano-TiO2 was also observed in S.oblignus,being found to promote the growth at low concentrations of 1 mg· L-1,which could increase the content of photosynthetic pigments,especially the content of the chlorophyll b and protein.As the concentration increased,the stimulative effect was reduced and the inhibition effect was enhanced.The influence on the activities of SOD and POD was consistent with that of cell growth.These studies suggested that nano-ZnO and nano-TiO2 at high concentrations induced toxicological effects.关键词
纳米ZnO/纳米TiO2/超氧化物歧化酶(SOD)/过氧化物酶(POD)/毒性效应Key words
nano-ZnO/ nano-TiO2/ superoxide dismutase(SOD)/ peroxides(POD)/ toxicity effect分类
资源环境引用本文复制引用
李雅洁,王静,崔益斌,李梅..纳米氧化锌和二氧化钛对斜生栅藻的毒性效应[J].农业环境科学学报,2013,32(6):1122-1127,6.基金项目
国家自然科学基金(21077052) （21077052）
江苏省社会发展项目(BE2012737) （BE2012737）
