农业环境科学学报2013，Vol.32Issue(6)：1143-1149,7.DOI:10.11654/jaes.2013.06.009
戊二醛胁迫对黑藻光合作用及抗氧化酶活性的影响
Effects of Glutaraldehyde Stress on Photosynthesis and Antioxidase Activity of Hydrilla Verticillata
摘要
Abstract
In order to evaluate the injury and inherent physiological mechanism of glutaraldehyde stress on advanced aquatic organisms,Hydrilla verticillata was cultivated in 1/20 Hoagland solution with 0 mg· L-1,1 mg· L-1,5 mg· L-1,10 and 50 mg· L-1 glutaraldehyde.Chlorophyll content,photosynthetic rate,antioxidase(SOD,POD and CAT) activity and MDA content were measured.The results showed that with the increase of concentration of glutaraldehyde and the extension of stress time,the content of chlorophyll a and chlorophyll b declined and the photosynthetic rate dropped.Under the treatment of 1 mg·L-1 or 5 mg·L-1 glutaraldehyde for 1 day and 3 days,SOD activity increased.But at 10 mg· L-1 or higher concentration of glutaraldehyde,it was significantly lower than that of the control.POD activity increased significantly under the treatment of 1 mg·L-1,5 mg· L-1 and 10 mg· L-1 glutaraldehyde for 1 day and 3 days,but when the stress time extended to 5 days,it showed an absolute dropping even at low concentration of 1 mg· L-1 glutaraldehyde.At a general level,during all treatments,CAT activity showed a higher level under 1 mg· L-1 glutaraldehyde,but 5 mg· L-1 or higher concentration of glutaraldehyde stress made CAT activity lower than that of the control.MDA content remained almost unchanged in 1 day stress of all glutaraldehyde concentrations.However,when the stress time prolonged to 3 days and longer,it showed a rising tendency as the glutaraldehyde concentration increased.Generally,the results showed that antioxidase activity of H.verticillata was stimulated by low concentration of glutaradehyde but declined with higher glutaradehyde concentration and prolonging stress time.It's worth noting that CAT activity did not decline with the treatment of 1 mg· L-1 glutaraldehyde for 7 days,suggesting CAT might be more tolerant to low content of glutaraldehyde than SOD and POD.关键词
戊二醛/黑藻/抗氧化酶活性/光合速率Key words
glutaraldehyde/ Hydrilla verticillata/ antioxidase activity/ photosynthetic rate分类
农业科技引用本文复制引用
胡一鸿,王梦龙,袁盛建,莫亿伟..戊二醛胁迫对黑藻光合作用及抗氧化酶活性的影响[J].农业环境科学学报,2013,32(6):1143-1149,7.基金项目
湖南人文科技学院博士启动基金(1007) （1007）
