农业环境科学学报2013，Vol.32Issue(6)：1178-1184,7.DOI:10.11654/jaes.2013.06.014
石油污染土壤生物修复对土壤酶活性的影响
Effect of Soft Enzyme Activities During Bioremediation of Crude Oil-Contaminated Soil
摘要
Abstract
Soil enzyme activity comprehensively reflects the soil microorganism biochemical process.In order to explore the changes of soil enzyme activities during bioremediation of crude oil-contaminated soil,constructed crude oil degrading bacterial consortium were built withthree strains isolated from oil-contaminated soil,bioremediation experiments of crude oil-contaminated soil were conducted,and the change of total petroleum hydrocarbon(TPH),apparent degradation effciencies and soil enzyme activities (urease,catalase,dehydrogenase and lipase) were examined during the crude oil-contaminated soil bioremediation.The results showed that the apparent degradation effciency of crude oil reached 64.4％ after 40 d of bioremediation.Urease activity rapidly increased during 0～24 d,then tended to be stable.The catalase activity and dehydrogenase activity increased in early stage then slightly declined.However,lipase activity had a rapid rise phase (0～ 16 d),then obviously slowed down.The results of statistical analysis showed that soil urease activity and TPH had significant negative relationship (-0.916,P＜0.05).Catalase and dehydrogenase activities were both significantly correlated with soil TPH concentration,and their correlation coefficients were-0.974(P＜0.01) and-0.969(P＜0.01),respectively.However,soil lipase activity did not have significant correlationship with TPH(P＞0.05).Therefore,soil enzyme activities are potential valuable indicators of oil biodegradation.关键词
土壤/石油/生物修复/土壤酶活性/总石油烃Key words
soil/ crude oil/ bioremediation/ soil enzyme activity/ total petroleum hydrocarbon分类
资源环境引用本文复制引用
王华金,朱能武,杨崇,党志,吴平霄..石油污染土壤生物修复对土壤酶活性的影响[J].农业环境科学学报,2013,32(6):1178-1184,7.基金项目
广东省自然科学基金团队项目(9351064101000001) （9351064101000001）
教育部新世纪优秀人才支持计划项目(NCET-11-0166) （NCET-11-0166）
