农业生物技术学报2013，Vol.21Issue(6)：698-706,9.
马铃薯腐烂茎线虫2-Cys型过氧化物还原酶基因的克隆及低剂量杀线虫剂对其表达的影响
Cloning and Sequence Analysis of a 2-cysteine Peroxiredoxin Gene from Ditylenchus destructor and the Effects of Low-dose Nematicides on Its Expression
摘要
Abstract
Peroxiredoxins(Prxs)are a superfamily of cysteine-dependent peroxidases that decompose hydrogen peroxide.Among the family,typical 2-cysteine Peroxiredoxins (2-Cys Prxs) are multifunctional proteins.In plant nematode,2-Cys Prxs play an important role in nematode development in planta or in countering radicals produced by the plant as a defense response to parasitism.In order to understand the effect of low dose of nematicides on 2-Cys Prxs mRNA expression,a novel 2-Cys Prx gene was cloned from Ditylenchus destructor using RT-PCR and RACE techniques,and its mRNA expression of D.destructor treated by low dose(10、1、0.1、0.01 、0.001 and 0.0001 μg/mL) nematieides of emanectin benzoate and aldicarb were monitored using Real-time PCR after 24 h.The results showed that the full-length cDNA was 784 bp with an open reading frame (ORF) of 591 bp encoding 196 amino acids residues,named Dd-prx-1(GenBank accession No.JQ609353).The protein had a predicted molecular weight of 21 850.09 D and a hypothetical pI of 6.83.The structure of Dd-Prx-1 genomic DNA include 6 extons and 5 introns.The N-terminal of predicted protein had no apparent signal peptide,but there was one transmembrane domain containing of 23 hydrophilic amino acids.Homology analysis showed that the deduced Dd-PRX-1 protein was highly homologous to the 2-cysteine peroxiredoxin in other nematodes.After treated by low dose emanectin benzoate and aldicarb,the expression levels of Dd-Prx-1 had no significant difference compared with control (P＜0.05).The results provide the useful information for further research mechanisms of nematodes response to low dose of nematicides.关键词
马铃薯腐烂茎线虫/过氧化物还原酶/杀线虫剂/基因表达Key words
Ditylenchus destructor/ Peroxiredoxin/ Nematicide/ Gene expression引用本文复制引用
何旭峰,彭焕,彭德良,黄文坤,贺文婷,丁中..马铃薯腐烂茎线虫2-Cys型过氧化物还原酶基因的克隆及低剂量杀线虫剂对其表达的影响[J].农业生物技术学报,2013,21(6):698-706,9.基金项目
国家自然科学基金(No.31071667) （No.31071667）
