农药学学报2013，Vol.15Issue(3)：286-292,7.DOI:10.3969/j.issn.1008-7303.2013.03.08
苹果树腐烂病内生拮抗放线菌A-2的鉴定及其活性评价
Identification of antagonistic endophytic actinomycetes A-2 and evaluation of its activity against Valsa mali
摘要
Abstract
An endophytic actinomycetes A-2 against Valsa mali was isolated from healthy apple tree branches.The antagonistic spectrum of strain A-2 and its inhibitory activity against the hyphal growth and conidial germination of V.mali were investigated by using plate growth rate,flat confrontation culture and slide method.In addition,the inhibitory activity of strain A-2 against V.mali in detached apple twigs was also determined.The results showed that the inhibition rate of A-2 fermentation supernatant on V.mali could reach more than 90％ and it had obvious antifungal activity against V.ambiens and other eight pathogenic fungi.Strain A-2 could induce alteration on hyphae morphology of V.mali,such as deformation and enlargement.Different dilution of strain A-2 fermentation supematants all showed obvious inhibitory activity against V.mali in detached twigs.The control efficacy was still above 70％ when the supernatant was diluted by 500 folds and used before and after inoculation of V.mali.Based on the cultural characteristics,physiological and biochemical properties as well as 16S rDNA sequencing,the strain A-2 was identified as Streptomyces cavourensis.Actinomycets A-2 could be developed new biological pesticide for control of V.mali.关键词
内生放线菌/卡伍尔链霉菌/拮抗作用/苹果树腐烂病菌Key words
endophytic actinomycetes/ Streptomyces cavourensis/ antagonistic activity / Valse mali分类
农业科技引用本文复制引用
张清明,王彩霞,王海艳,李保华,董向丽,李桂舫..苹果树腐烂病内生拮抗放线菌A-2的鉴定及其活性评价[J].农药学学报,2013,15(3):286-292,7.基金项目
山东省科技攻关计划项目(2010GNC10918) （2010GNC10918）
现代农业产业技术体系(CARS-28) （CARS-28）
国家自然科学基金项目(31000891,31272001) （31000891,31272001）
山东省"泰山学者"建设工程项目. （）
