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橡胶树三倍体种质创制及生物学鉴定

赖杭桂 陈霞 徐洪伟 吴繁花 唐燕琼 胡新文

热带作物学报2013,Vol.34Issue(6):1001-1006,6.
热带作物学报2013,Vol.34Issue(6):1001-1006,6.DOI:10.3969/j.issn.1000-2561.2013.06.001

橡胶树三倍体种质创制及生物学鉴定

Creation and Biological Identification of Triploid Germplasm in Rubber Tree

赖杭桂 1陈霞 1徐洪伟 1吴繁花 1唐燕琼 1胡新文1

作者信息

  • 1. 海南大学农学院,海南海口 570228
  • 折叠

摘要

Abstract

In this research,the triploid germplasm of rubber tree was artificially induced and identified by the morphological,cytological and molecular biological methods.The results showed that the homozygous triploid rubber gerrnplasm was obtained by hybridizing the diploid gamate (2n)induced during reproductive phase with normal haploid gamate(1n),and the asexual offspring was stable in ploidy.The chromosome number in the somatic cells of the offspring was 2n=3x=54,and the percentage of triploid cells was up to 91.5%.The results from the RAPD and POD showed that the triploid rubber germplasm had great affiliation with Reyan 7-33-97 and RRIM600.It could be concluded that Reyan 7-33-97 and RRIM600 were the crossing parents of this triploid rubber germplasm..

关键词

橡胶树/三倍体种质/RAPD/过氧化物同工酶

Key words

Rubber tree/Triploid germplasm/RAPD/Peroxide isoenzyme (POD)

分类

农业科技

引用本文复制引用

赖杭桂,陈霞,徐洪伟,吴繁花,唐燕琼,胡新文..橡胶树三倍体种质创制及生物学鉴定[J].热带作物学报,2013,34(6):1001-1006,6.

基金项目

教育部热带作物新品种选育工程中心和作物学重点学科联合资助项目(No.1hxm-2012-4). (No.1hxm-2012-4)

热带作物学报

OA北大核心CSCDCSTPCD

1000-2561

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