热带作物学报2013，Vol.34Issue(6)：1007-1012,6.DOI:10.3969/j.issn.1000-2561.2013.06.002
根癌农杆菌介导柱花草炭疽菌遗传转化体系的优化
Optimization of Genetic Transformation System of Stylo Anthracnose Mediated by Agrobacterium tumefaciens
摘要
Abstract
In this study,an Agrobacterium tumefaciens AGL-1 containing binary vector with ILV1 and GFP as report genes was used for transformation of Colletotrichum gloeosporioides.In order to gain higher transformation efficiency,the major facts affecting transformation efficiency was analyzed by a one-factor experiment and a twofactor experiment.The optimization of genetic transformation system of C.gloeosporioides was established,and we optimized the conditions of transformed system.The transformation efficiency was up to 300 to 400 transformants per 106 conidia.All the transformants tested had GFP gene comfirmed by PCR,and GFP signals were also detected.The study provided a basis for further study on the pathogenesis of C.gloeosporioides,optimized transformation system offered highly efficient means for generating an ATMT library of C.gloeosporioides.And it would help to analyze the function of pathogenicity gene of this pathogen.关键词
柱花草/炭疽菌/遗传转化Key words
Stylosanthes guianensias SW/ Colletotrichum gloeosporioides/ Genetic transformation分类
生物科学引用本文复制引用
胡彩平,郑金龙,高建明,蔡志英,黄贵修,习金根,张世清,陈河龙,易克贤..根癌农杆菌介导柱花草炭疽菌遗传转化体系的优化[J].热带作物学报,2013,34(6):1007-1012,6.基金项目
国家自然科学基金项目"柱花草炭疽菌致病相关基因的克隆及功能分析"(No.31072076) （No.31072076）
海南大学211工程建设项目. （）
