热带作物学报2013，Vol.34Issue(6)：1082-1089,8.DOI:10.3969/j.issn.1000-2561.2013.06.016
木薯苹果酸脱氢酶基因克隆和表达分析
Cloning and Expression Analysis of Malate Dehydrogenase Gene from Cassava
摘要
Abstract
A 1 175 bp cDNA sequence of MDH with a 999 bp open reading frame,encoding a protein with 332 amino acids,was obtained from cassava SC8 roots by RACE (rapid-amplification of cDNA ends) to study the expressional pattern of malate dehydrogenase (MDH) mRNA.The MDH shared 84％～94％ of amino acid sequence identities with MDH from other species,and contained a typical NAD+ binding motif (TGAAGQI) and a catalytic motif (IWGNH),suggesting it was a cytosolic molate dehydrogenase (cyMDH) gene.MDH was perhaps involved in the process of starch accumulation in cassava,the expression of cassava MDH was up regulated during storage root thinckening.关键词
木薯/块根/苹果酸脱氢酶/淀粉合成Key words
Manihot esculenta Crantz./ Tuberous roots/ Malate dehydrogenase/ Starch metabolism分类
农业科技引用本文复制引用
尹奇,仝征,贺庭琪,王力敏,黄启星,郭运玲,孔华,王旭初,郭安平..木薯苹果酸脱氢酶基因克隆和表达分析[J].热带作物学报,2013,34(6):1082-1089,8.基金项目
国家重点基础研究发展973计划(No.2010CB126605) （No.2010CB126605）
中国热带农业科学院热带生物技术研究所基本科研业务费专项(No.ITBB110101,No.ITBB110102) （No.ITBB110101,No.ITBB110102）
海南省自然科学基金(No.310080). （No.310080）
