热带作物学报2013，Vol.34Issue(6)：1098-1102,5.DOI:10.3969/j.issn.1000-2561.2013.06.018
香蕉泛素结合酶基因的克隆及其功能初步分析
Cloning and Expression Analysis of mauce2 Gene from Banana's Root
摘要
Abstract
A UCE gene named as mauce2 was obtained by RACE technology based on a fragment from banana's root cDNA library.The full length of the gene was 929 bp,containing an integrated ORF encoding 148 amino acids.Molecular weight and isoelectric point of the protein was 16 505.1 ku and 8.41.Amino acids homology analysis indicated that the sequence had 97.97％,97.30％,96.62％,95.95％ similarity with Selaginella moellendorffii,Glycine max,Malus × domestica,Arabidopsis thaliana RT-PCR analysis showed that mauce2 was ubiquitously expressed in roots,stems,leaves,flowers and fruits,and the expression level was higher in flower and fruit than in leaves.关键词
香蕉/泛素结合酶基因/克隆/实时定量PCR/组织特异性表达分析Key words
Banana/ Ubiquitin conjugating enzyme/ Cloning/ Real-time quantitative PCR/ Expression analysis分类
农业科技引用本文复制引用
李羽佳,金志强,刘菊华,贾彩红,张建斌,王甲水,许奕,徐碧玉..香蕉泛素结合酶基因的克隆及其功能初步分析[J].热带作物学报,2013,34(6):1098-1102,5.基金项目
中央级公益性科研院所基本科研业务费专项资金(No.ITBB110202) （No.ITBB110202）
国家现代香蕉产业技术体系(No.CARS-32). （No.CARS-32）
