热带作物学报2013,Vol.34Issue(6):1110-1114,5.DOI:10.3969/j.issn.1000-2561.2013.06.020
几种常见蝴蝶兰组培突变体的RAPD检测
Detection of Several Common Phalaenopsis Somaclonal Mutants by RAPD
摘要
Abstract
Three somaclonal mutants,namely unsymmetrical flower with incised petal and/or sepal,peloric flower with all three petals as the labellum,and chimeric mutation in Phalaenopsis flower color,were screened.All the mutants were compared and analyzed with the control(wild-type Phalaenopsis)by RAPD using 50 UBC arbitrary primers.The results showed that RAPD analysis was useful for the mutants with unsymmetrical flower and the mutants with chimeric flowers,but not the other kind of mutant.Further,different incised mutant had different polymorphic fragments with wide individual variability.关键词
蝴蝶兰/突变体/RAPDKey words
Phalaenopsis/Mutant/ RAPD分类
农业科技引用本文复制引用
肖文芳,李佐,尤毅,陈和明,吕复兵..几种常见蝴蝶兰组培突变体的RAPD检测[J].热带作物学报,2013,34(6):1110-1114,5.基金项目
广东省现代农业产业技术体系建设专项 ()
广州市农业科技项目(No.GZCQC1002FG08015-01) (No.GZCQC1002FG08015-01)
广东省教育部产学研结合项目(No.2011B090400239). (No.2011B090400239)