热带作物学报2013，Vol.34Issue(6)：1176-1181,6.DOI:10.3969/j.issn.1000-2561.2013.06.032
石蒜生物碱的双向薄层色谱分析及气质联用鉴定
The Analysis of Lycoris radiata Alkaloids by Two-Dimensional TLC and Identification by GC-MS
摘要
Abstract
A method for the identification and preliminary preparation of L.radiata alkaloids was established.The one-dimensional and two-dimensional thin-layer chromatography (TLC) analysis were used to separate the alkaloids of L.radiata.The results showed that the optimized eluent was CHCI3/MeOH/NH3·H2O (9 ∶ 1 ∶ 0.2,V/V/V) for onedimensional TLC.The optimized eluent was EtOAc/MeOH/NH3·H2O (9∶1∶0.2,V/V/V) in first direction and CHCl3/MeOH/NH3·H2O (9∶1∶0.2,V/V/V) in the second direction for two-dimensional TLC.The two-dimensional TLC exhibited a better seperation effect than the one-dimensional TLC.Applied the two-dimensional TLC,16 spots were seperated.The method was used to analyze the alkaloids in different parts of L.radiata,which showed that alkaloids types and amounts were the highest in roots,followed by bulbs,and finally leaves.Applied the method,the seperated 16 spots were detected by HPLC.The result showed that 15 alkaloids chromatographic peaks were obvious and the purities of 8 kinds were above 90％,of which the purities above 93％ detected by GC-MS were lycorine,lycoramine,galanthamine,tazettine,pluviine.The method was suitable for analyzing and preparing L.radiata alkaloids.关键词
薄层色谱/石蒜/生物碱/HPLC/GC-MSKey words
TLC/ Lycoris radiata/ Alkaloid/ HPLC/ GC-MS分类
医药卫生引用本文复制引用
张玉琼,高翠云,朱景存,董召荣,陈娜..石蒜生物碱的双向薄层色谱分析及气质联用鉴定[J].热带作物学报,2013,34(6):1176-1181,6.基金项目
安徽省自然科学基金(No.1208085mc48) （No.1208085mc48）
国家公益性行业(农业)科研专项(No.201103001,201203032,201203096). （农业）
