水产学报2013，Vol.37Issue(5)：661-671,11.DOI:10.3724/SP.J.1231.2013.38371
基于中国明对虾不同组织EST数据的基因表达差异分析
Differential gene expression analysis based on expressed sequence tags(EST) from different tissues of Fenneropenaeus chinensis
摘要
Abstract
Bioinformatics analysis was conducted based on the acquired expressed sequence tags(ESTs) data from different tissues of Fenneropenaeus chinensis.A total of 10 446,2 690,1 067 and 1 282 original ESTs were obtained from cephalothorax,blood,eyestalk,and ovary of adult F.chinensis respectively.By clustering and assembling,3 454,1 053,406 and 544 unigenes were generated,and then annotated by searching in NR,GO,KEGG databases.The tissue specific transcripts were identified through sequence homology analysis and classified by GO annotation.The result indicated that specific transcripts in blood group were specifically enriched in the GO term of virion part,locomotion,rhythmic process,cell killing and multi-organism process.Specific transcripts in eyestalk group were specifically enriched in the GO term of molecular transducer activity,response to stimulus,multicellular organismal process,pigmentation and biological regulation.Specific transcripts in ovary group were specifically enriched in the GO term of nutrient reservoir activity,reproduction,anatomical structure formation,transporter activity and establishment of localization.We also analyzed the highly expressed genes in each tissue according to the number of ESTs of each unigene.The result indicated that genes encoding peritrophin,elongation factor 1-alpha,thrombospondin and arginine kinase were widespread and highly expressed in tissues,suggesting that they were involved in a variety of important biological processes in shrimp.In addition,genes encoding penaeidin,cytochrome c oxidase and 14-3-3-like protein were highly expressed in blood group and genes encoding arrestin and rhodopsin were highly expressed in eyestalk group.For understanding the expressions of common genes,we analyzed genes related to peritrophin and peroxiredoxin,revealing that genes of peritrophin in F.chinensis were multiform and highly expressed.This result may provide reference for further functional study of peritrophin.关键词
中国明对虾/表达序列标签(EST)/组织差异/组织高表达基因Key words
Fenneropenaeus chinensis/ expressed sequence tags (EST)/ tissue variability/ tissue highly expressed genes分类
生物科学引用本文复制引用
隗健凯,柳承璋,张晓军,王兵,董波,李富花,相建海..基于中国明对虾不同组织EST数据的基因表达差异分析[J].水产学报,2013,37(5):661-671,11.基金项目
国家"八六三"高技术研究发展计划(2012AA10A404,2012AA092205) （2012AA10A404,2012AA092205）
国家自然科学基金项目(30972245,41076101,31172396) （30972245,41076101,31172396）
