水产学报2013，Vol.37Issue(6)：816-822,7.DOI:10.3724/SP.J.1231.2013.38343
三疣梭子蟹Ⅰ型微卫星标记的发掘及多态性分析
Identification of type Ⅰ microsatellite markers and their polymorphism in Portunus trituberculatus
摘要
Abstract
In the present study,type Ⅰ microsatellites of Portunus trituberculatus were screened from 13 985 ESTs in the NCBI database by bioinformatics approach.A total of 287 type Ⅰ microsatellites were identified,among which 173 were the type of dinucleotide repeat being the most abundant microsatellite and accounting for 60.3％ of the identified microsatellite markers,and 79 were the type of trinucleotide accounting for 27.5％,while hexa-,penta-and tetra-nucleotide repeats were rare.Among the dinucleotide repeats,AC/GT was the most abundant type with percentage of 53.8％,followed by the AG/CT type with 37.0％,while AT and GC were rare.14 microsatellites were selected for polymorphism analyses,8 of them showed polymorphism in a wild population of P.trituberculatus.The mean PIC and heterozygosity values were 0.57 and 0.63,respectively.Among them,6 PIC of microsatellites greater than 0.5,showing high polymorphism.This study showed that the way of screening type Ⅰ microsatellite markers from EST data was feasible.These type Ⅰ microsatellites can be used for further study in genetic diversity,genetic mapping and quantitative trait loci(QTLs) identification in P.trituberculatus.关键词
三疣梭子蟹/微卫星标记/多态信息含量Key words
Portunus trituberculatus/microsatellite/polymorphism information content(PIC)分类
生物科学引用本文复制引用
吕建建,王渝,高保全,李健,刘萍..三疣梭子蟹Ⅰ型微卫星标记的发掘及多态性分析[J].水产学报,2013,37(6):816-822,7.基金项目
国家"八六三"高技术研究发展计划(2012AA10A409) （2012AA10A409）
中国博士后科学基金(2013M531657) （2013M531657）
黄海水产研究所基本科研业务费专项(20603022013039) （20603022013039）
